Advanced Methods in Molecular Biology and Biotechnology

Advanced Methods in Molecular Biology and Biotechnology
Author: Khalid Z. Masoodi,Sameena Maqbool Lone,Rovidha Saba Rasool
Publsiher: Academic Press
Total Pages: 248
Release: 2020-11-15
ISBN: 0128244496
Category: Science
Language: EN, FR, DE, ES & NL

Advanced Methods in Molecular Biology and Biotechnology Book Excerpt:

Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology Features clear, step-by-step instruction for applying the techniques covered Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment

Advanced Methods in Molecular Biology and Biotechnology

Advanced Methods in Molecular Biology and Biotechnology
Author: Khalid Z. Masoodi,Sameena Maqbool Lone,Rovidha Saba Rasool
Publsiher: Academic Press
Total Pages: 218
Release: 2020-11-10
ISBN: 012824450X
Category: Science
Language: EN, FR, DE, ES & NL

Advanced Methods in Molecular Biology and Biotechnology Book Excerpt:

Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology Features clear, step-by-step instruction for applying the techniques covered Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment

Advanced Methods in Molecular Systems Biology

Advanced Methods in Molecular Systems Biology
Author: Andreas Dress
Publsiher: Unknown
Total Pages: 5
Release: 2010
ISBN: 1928374650XXX
Category: Electronic Book
Language: EN, FR, DE, ES & NL

Advanced Methods in Molecular Systems Biology Book Excerpt:

Molecular Biology Techniques

Molecular Biology Techniques
Author: Sue Carson,Susan Carson,Heather Miller,D. Scott Witherow
Publsiher: Academic Press
Total Pages: 200
Release: 2012
ISBN: 0123855446
Category: Medical
Language: EN, FR, DE, ES & NL

Molecular Biology Techniques Book Excerpt:

This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The "project" approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Practical Techniques in Molecular Biotechnology

Practical Techniques in Molecular Biotechnology
Author: Bal Ram Singh,Raj Kumar
Publsiher: Cambridge University Press
Total Pages: 500
Release: 2021-10-31
ISBN: 1108486401
Category: Medical
Language: EN, FR, DE, ES & NL

Practical Techniques in Molecular Biotechnology Book Excerpt:

The book will be useful for undergraduate students as a supplementary/reference text in the field of molecular biotechnology.

Advances in Molecular Techniques

Advances in Molecular Techniques
Author: Rakesh S. Sengar,Reshu Chaudhary
Publsiher: CRC Press
Total Pages: 474
Release: 2018-05-14
ISBN: 9780815370758
Category: Medical
Language: EN, FR, DE, ES & NL

Advances in Molecular Techniques Book Excerpt:

Molecular genetics aims to comprehend biological activity at the gene sub-level. Scientists from different areas of research and applied science can use the standard techniques optimized by molecular biologists.This book serves as a guide that introduces classic molecular biology techniques and advances in molecular and genetic engineering.

Bioinformatics for Everyone

Bioinformatics for Everyone
Author: Mohammad Yaseen Sofi,Afshana Shafi,Khalid Z. Masoodi
Publsiher: Academic Press
Total Pages: 266
Release: 2021-09-14
ISBN: 0323911293
Category: Business & Economics
Language: EN, FR, DE, ES & NL

Bioinformatics for Everyone Book Excerpt:

Bioinformatics for Everyone provides a brief overview on currently used technologies in the field of bioinformatics—interpreted as the application of information science to biology— including various online and offline bioinformatics tools and softwares. The book presents valuable knowledge in a simplified way to help students and researchers easily apply bioinformatics tools and approaches to their research and lab routines. Several protocols and case studies that can be reproduced by readers to suit their needs are also included. Explains the most relevant bioinformatics tools available in a didactic manner so that readers can easily apply them to their research Includes several protocols that can be used in different types of research work or in lab routines Discusses upcoming technologies and their impact on biological/biomedical sciences

Methods in Plant Biochemistry and Molecular Biology

Methods in Plant Biochemistry and Molecular Biology
Author: William V. Dashek
Publsiher: CRC Press
Total Pages: 480
Release: 2017-11-29
ISBN: 9781315895383
Category: Electronic Book
Language: EN, FR, DE, ES & NL

Methods in Plant Biochemistry and Molecular Biology Book Excerpt:

Modern plant science research currently integrates biochemistry and molecular biology. This book highlights recent trends in plant biotechnology and molecular genetics, serving as a working manual for scientists in academic, industrial, and federal laboratories. A wide variety of authors have contributed to this book, reflecting the thinking and expertise of active investigators who generate advances in technology. The authors were selected especially for their ability to create and/or implement novel research methods.

The Chloroplast From Molecular Biology to Biotechnology

The Chloroplast  From Molecular Biology to Biotechnology
Author: Joan H. Argyroudi-Akoyunoglou,Horst Senger
Publsiher: Springer Science & Business Media
Total Pages: 356
Release: 1999-02-28
ISBN: 9780792355762
Category: Science
Language: EN, FR, DE, ES & NL

The Chloroplast From Molecular Biology to Biotechnology Book Excerpt:

From August 10 to August 15, 1998, an international Advanced Research Workshop-Lecture Course on The chloroplast: from Molecular Biology to Biotechnology was held at the Orthodox Academy of Crete, Kolymbari-Chania, on the island of Crete, Greece. After five previous meetings on the chloroplast topic in Marburg (1975), Spetses (1978), Rhodos (1985), Aghia Pelaghia, Crete (1991) and Marburg (1995) this conference proved again that chloroplast research is continuously in the focus of intensive research interest. The meeting, sponsored by NATO and supported by the Federation of the European Societies for Plant Physiology (FESPP) and the Greek Ministry of Development (General Secretariat of Research and Development), was held under the auspices of the International Society for Chloroplast Development and the National Center for Scientific Research "Demokritos", Athens-Greece. Aim of the workshop was to bring together experts and students from different disciplines, coming from various countries around the world, studying chloroplast biogenesis from different perspectives in an effort to propose biotechnological approaches, via genetic manipulation of the organelle, applicable in solving problems of economic importance. Ninety scientists (including observers) coming from 19 countries actively participated and discussed recent advances in the field. During the meeting it became clear that as a result of the progress made in molecular biology, including genomic sequence, and in biochemistry over recent years, this exciting field of chloroplast development is continuously promoted by renewed interest in as yet unsolved but very important questions.

Advanced Biotechnology

Advanced Biotechnology
Author: R C Dubey
Publsiher: S. Chand Publishing
Total Pages: 1161
Release: 2014
ISBN: 812194290X
Category: Science
Language: EN, FR, DE, ES & NL

Advanced Biotechnology Book Excerpt:

The book embodies 22 chapters covering various important disciplines of biotechnology, such as cell biology, molecular biology, molecular genetics, biophysical methods, genomics and proteomics, metagenomics, enzyme technology, immune-technology, transgenic plants and animals, industrial microbiology and environmental biotechnology. The book is illustrative. It is written in a simple language

Advanced Methods in Protein Microsequence Analysis

Advanced Methods in Protein Microsequence Analysis
Author: Brigitte Wittmann-Liebold,Johann Salnikow
Publsiher: Springer Science & Business Media
Total Pages: 424
Release: 2012-12-06
ISBN: 3642715346
Category: Science
Language: EN, FR, DE, ES & NL

Advanced Methods in Protein Microsequence Analysis Book Excerpt:

Much of the recent spectacular progress in the biological sciences can be at tributed ot the ability to isolate, analyze, and structurally characterize proteins and peptides which are present in cells and cellular organelles in only very small amounts. Recent advances in protein chemistry and in particular the application of new micromethods have led to fruitful advances in the understanding of basic cellular processes. Areas where protein-chemical studies have resulted in interest ing discoveries include the peptide hormones and their release factors, growth factors and oncogenes, bioenergetics, proton pumps and ion pumps and chan nels, topogenesis and protein secretion, molecular virology and immunology, membrane protein analysis, and receptor research. In fact, the key methods are now on hand to unravel many of the major outstanding problems of molecular biology and in particular questions of fundamental interest which relate to devel opmental biology and specificity in cell-cell interaction. In this volume we have assembled descriptions of procedures which have re cently been shown to be efficaceous for the isolation, purification, and chemical characterization of proteins and peptides that are only available in minute amounts. Emphasis is placed on well-established micromethods which have been tested and found useful in many laboratories by experienced investigators. The chapters are written by specialists, and describe a range of sensitive techniques which can be used by researchers working in laboratories with only modest resources and equipment.

Patenting of Human Genes and Living Organisms

Patenting of Human Genes and Living Organisms
Author: Friedrich Vogel,Reinhard Grunwald
Publsiher: Springer Science & Business Media
Total Pages: 244
Release: 2012-12-06
ISBN: 3642851533
Category: Medical
Language: EN, FR, DE, ES & NL

Patenting of Human Genes and Living Organisms Book Excerpt:

Biotechnology is one of the most promising fields of technology, especially since molecular biology methods have enhanced our knowledge of genes, their structure, and their action. This knowledge makes it possible to change genetic material and construct new varieties of cultural plants and animals for various purposes such as nutrition, scientific and medical experimentation, and treatment of human diseases. Such inventions may even include human genes. The understandable desire to have legal protection in this domain has created new problems - especially from the viewpoint of the law and acquiring patents for these new inventions. These problems are under wide discussion and are often controversial. This volume provides a unique overview of the current problems and opinions in this controversial field.

Rice Biotechnology

Rice Biotechnology
Author: Gurdev S. Khush,Gary H. Toenniessen
Publsiher: Int. Rice Res. Inst.
Total Pages: 320
Release: 1991-01-01
ISBN: 0851987125
Category: Science
Language: EN, FR, DE, ES & NL

Rice Biotechnology Book Excerpt:

The world rice economy: challenges ahead; Research priorities for rice biotechnology; Genetic diversity of wild and cultivated rice; Rice karyotype, marker genes, and linkage groups; Development and use of restriction fragment length polymorphism in rice breeding and genetics; Rice tissue culture and its application; Transformation and regeneration of rice protoplasts; Assessment of rice genetic transformation techniques; The identification and characterization of rice nuclear genes; Gene expression in rice; Potentially useful genes for rice genetic engineering; Molecular probes for isease diagnosis and monitoring; Prospects for the future.

Computational Methods in Molecular Biology

Computational Methods in Molecular Biology
Author: S.L. Salzberg,D.B. Searls,S. Kasif
Publsiher: Elsevier
Total Pages: 368
Release: 1998-06-19
ISBN: 9780080860930
Category: Computers
Language: EN, FR, DE, ES & NL

Computational Methods in Molecular Biology Book Excerpt:

Computational biology is a rapidly expanding field, and the number and variety of computational methods used for DNA and protein sequence analysis is growing every day. These algorithms are extremely valuable to biotechnology companies and to researchers and teachers in universities. This book explains the latest computer technology for analyzing DNA, RNA, and protein sequences. Clear and easy to follow, designed specifically for the non-computer scientist, it will help biologists make better choices on which algorithm to use. New techniques and demonstrations are elucidated, as are state-of-the-art problems, and more advanced material on the latest algorithms. The primary audience for this volume are molecular biologists working either in biotechnology companies or academic research environments, individual researchers and the institutions they work for, and students. Any biologist who relies on computers should want this book. A secondary audience will be computer scientists developing techniques with applications in biology. An excellent reference for leading techniques, it will also help introduce computer scientists to the biology problems. This is an outstanding work which will be ideal for the increasing number of scientists moving into computational biology.

Laboratory Production of Cattle Embryos

Laboratory Production of Cattle Embryos
Author: Ian Gordon
Publsiher: CABI
Total Pages: 548
Release: 2003-01-01
ISBN: 9780851998480
Category: Technology & Engineering
Language: EN, FR, DE, ES & NL

Laboratory Production of Cattle Embryos Book Excerpt:

3000 new references added since the first editionGives information necessary to produce embryos totally through in vitro techniques Shows commercial applications of embryo and oocyte researchCattle remain at the forefront of many new developments in reproductive technology and what can be done for the cow today will later be applicable to other farm livestock and perhaps humans. This new edition reviews the considerable advances and issues in embryo production technology, based on reports since the first edition in 1994. This is a must have volume for those who own the first edition, and in itself an incredibly informative text.

Protein Downstream Processing

Protein Downstream Processing
Author: Nikolaos E. Labrou
Publsiher: Unknown
Total Pages: 555
Release: 2014
ISBN: 9781627039772
Category: Biochemistry
Language: EN, FR, DE, ES & NL

Protein Downstream Processing Book Excerpt:

Proteins are the most diverse group of biologically important substances. With the recent technological advances in the genomics area and the efforts in proteomics research, the rate of discovery for new proteins with unknown structure and function has increased. These proteins generated from genomic approaches present enormous opportunities for research and industrial application. Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods is a compilation of chapters within the exciting area of protein purification designed to give the laboratory worker the information needed to design and implement a successful purification strategy. It presents reliable and robust protocols in a concise form, emphasizing the critical aspects on practical problems and questions encountered at the lab bench. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Protein Downstream Processing: Design, Development and Application of High and Low-Resolution Methods will be an ideal source of scientific information to advanced students, junior researchers, and scientists involved in health sciences, cellular and molecular biology, biochemistry, and biotechnology and other related areas in both academia and industry.

Advanced Techniques in Soil Microbiology

Advanced Techniques in Soil Microbiology
Author: Ajit Varma,Ralf Oelmüller
Publsiher: Springer Science & Business Media
Total Pages: 427
Release: 2007-07-25
ISBN: 3540708650
Category: Science
Language: EN, FR, DE, ES & NL

Advanced Techniques in Soil Microbiology Book Excerpt:

This book presents a wide range of biotechnological methods for application in soil microbiology analysis, including all essential methods involving molecular biology, immunology, microbiology, and structural biology, such as transcriptome analysis, RNAi technology, molecular matchmaking, RAPD, T-RFLP and FT/MS. The techniques and procedures presented here offer practical guides for immediate use in the laboratory. This volume will be of use both to the first-timer and to the experienced scientist.

Advanced Methods in Plant Breeding and Biotechnology

Advanced Methods in Plant Breeding and Biotechnology
Author: David Ronald Murray
Publsiher: C A B International
Total Pages: 392
Release: 1991
ISBN: 1928374650XXX
Category: Science
Language: EN, FR, DE, ES & NL

Advanced Methods in Plant Breeding and Biotechnology Book Excerpt:

Breeding plants for the twenty-first century; Chromosomal organization and gene mapping; Gene transfer to plants using Agrobacterium; Electroporation for direct gene transfer into plant protoplasts; Microprojectile techniques for direct gene transfer into intact plant cells; Localization of transferred genes in genetically modifically modified plants; Somatic embryogenesis: potential for use in propagation and gene transfer systems; Chloroplast and mitochondrial genomes: manipulation through somatic hybridization; Modification of the chloroplast genome with particular reference to herbicide resistance; Breeding for resistance to insects; Resistance to fungal diseases; Advances in breeding for resistance to bacterial pathogens; Genetic engineering for resistance to viruses; Breeding for resistance to physiological stresses.

NIH Reauthorization and Protection of Health Facilities

NIH Reauthorization and Protection of Health Facilities
Author: United States. Congress. House. Committee on Energy and Commerce. Subcommittee on Health and the Environment
Publsiher: Unknown
Total Pages: 362
Release: 1991
ISBN: 1928374650XXX
Category: Animal experimentation
Language: EN, FR, DE, ES & NL

NIH Reauthorization and Protection of Health Facilities Book Excerpt:

Departments of Labor Health and Human Services Education and Related Agencies Appropriations for Fiscal Year 1990 Department of Health and Human Services

Departments of Labor  Health and Human Services  Education  and Related Agencies Appropriations for Fiscal Year 1990  Department of Health and Human Services
Author: United States. Congress. Senate. Committee on Appropriations. Subcommittee on Departments of Labor, Health and Human Services, Education, and Related Agencies
Publsiher: Unknown
Total Pages: 1302
Release: 1990
ISBN: 1928374650XXX
Category: United States
Language: EN, FR, DE, ES & NL

Departments of Labor Health and Human Services Education and Related Agencies Appropriations for Fiscal Year 1990 Department of Health and Human Services Book Excerpt: