Genome Editing

Genome Editing
Author: Kiran Musunuru
Publsiher: Academic Press
Total Pages: 230
Release: 2021-03-05
ISBN: 0128236477
Category: Medical
Language: EN, FR, DE, ES & NL

Genome Editing Book Excerpt:

Genome Editing: A Practical Guide to Research and Clinical Applications is geared towards investigators interested in learning how to use CRISPR-Cas9-based technologies, with a focus on cardiovascular research and clinical applications. Covering a range of topics from the basics of genome editing to design considerations, to assessments and applications, this reference allows readers to get started and establish a full workflow from the beginning of the project to its full completion. With worked examples drawn from real-life experiments, as well as troubleshooting and pitfalls to avoid, the book serves as an essential reference for researchers and investigators in both cardiovascular and biomedical research. Help readers familiarise with the variety of genome-editing approaches that are being applied in cardiovascular research and medicine, i.e., both research applications and clinical applications Understand the use of genome editing through worked examples (based on real-life experiments) in which CRISPR-Cas9 is employed, online tools to design CRISPR-Cas9 reagents, methods to interpret data from genome-editing experiments, the downsides of genome-editing technology - both the scientific and ethical pitfalls to avoid Written in an easy-to-follow manner, guiding readers from the design of the project to its completion Includes unpublished and new methods

Genome Editing

Genome Editing
Author: Kursad Turksen
Publsiher: Springer
Total Pages: 166
Release: 2016-08-09
ISBN: 3319341480
Category: Science
Language: EN, FR, DE, ES & NL

Genome Editing Book Excerpt:

This timely volume explores the use of CRISPR-Cas9 for genome editing, presenting cutting-edge techniques and their applications in treatment of disease. The chapters describe latest methods such as use of targetable nucleases, investigation of the non-coding genome, mouse genome editing, increasing of knock-in efficiency in mouse zygotes, and generation of reporter stem cells; the text contextualizes these methods in treatment of cardiovascular disease, diabetes mellitus, retinitis pigmentosa, and others. The final chapters round out the book with a discussion of controversies and future directions. Genome Editing is an essential, of-the-moment contribution to this rapidly growing field. Drawing from a wealth of international perspectives, it presents novel techniques and applications for the engineering of the human genome. This book is essential reading for all clinicians and researchers in stem cells, regenerative medicine, genomics, biochemical and biomedical engineering- especially those interested in learning more about genome editing and applying it in a targeted, specific way.

Isogenic Human Pluripotent Stem Cell Models of Cardiovascular Disease Associated Genetic Variation

Isogenic Human Pluripotent Stem Cell Models of Cardiovascular Disease Associated Genetic Variation
Author: Derek Tilghman Peters
Publsiher: Unknown
Total Pages: 135
Release: 2016
ISBN: 1928374650XXX
Category: Electronic Book
Language: EN, FR, DE, ES & NL

Isogenic Human Pluripotent Stem Cell Models of Cardiovascular Disease Associated Genetic Variation Book Excerpt:

The properties of human pluripotent stem cells (hPSCs) make them uniquely suited to address this challenge - they retain a normal human genome in culture, can be genetically modified, and can be differentiated into multiple cell types. The work presented in this thesis demonstrates the use of hPSCs and genome editing technology to generate human disease models in vitro. We developed a genome editing system optimized for hPSCs that can be used to efficiently generate hPSC lines with targeted genetic modifications. Modified and isogenic control hPSCs are then differentiated into a relevant cell type for phenotypic characterization. We applied this approach to investigate the functional effects of human genetic variation underlying plasma lipid concentrations.

Clinical and Molecular Aspects of Cardiomyopathies On the road from gene to therapy An Issue of Heart Failure Clinics E Book

Clinical and Molecular Aspects of Cardiomyopathies  On the road from gene to therapy  An Issue of Heart Failure Clinics  E Book
Author: Giuseppe Limongelli
Publsiher: Elsevier Health Sciences
Total Pages: 135
Release: 2018-04-02
ISBN: 0323610471
Category: Medical
Language: EN, FR, DE, ES & NL

Clinical and Molecular Aspects of Cardiomyopathies On the road from gene to therapy An Issue of Heart Failure Clinics E Book Book Excerpt:

This issue of Heart Failure Clinics--edited by Drs. Giuseppe Limongelli, Sharlene Day, and Perry Elliott--will cover Clinical and Molecular Aspects of Cardiomyopathies: On the Road from Gene to Therapy. Topics include, but are not limited to, Epidemiology and Clinical aspects of Genetic Cardiomyopathies, Existing and New therapies, Gene editing and gene-based therapeutics, Controversies surrounding exercise in genetic cardiomyopathies, Genetic infiltrative cardiomyopathies, LV Noncompaction, Clinical presentation and natural history of hypertrophic cardiomyopathy in Rasopathies, and Moelcular basis and new treatments of cardiac diseases in Rasopathies.

Genome Editing Applications in Animal Research

Genome Editing Applications in Animal Research
Author: Jun Song,Bradford Daigneault,Zhanjun Li,Huaqiang Yang
Publsiher: Frontiers Media SA
Total Pages: 135
Release: 2022-02-18
ISBN: 2889744426
Category: Science
Language: EN, FR, DE, ES & NL

Genome Editing Applications in Animal Research Book Excerpt:

Reprogramming the Genome CRISPR Cas based Human Disease Therapy

Reprogramming the Genome  CRISPR Cas based Human Disease Therapy
Author: Anonim
Publsiher: Academic Press
Total Pages: 390
Release: 2021-06-12
ISBN: 0323853242
Category: Science
Language: EN, FR, DE, ES & NL

Reprogramming the Genome CRISPR Cas based Human Disease Therapy Book Excerpt:

Reprogramming the Genome: CRISPR-Cas-based Human Disease Therapy, presents the collation of chapters written by eminent scientists worldwide. CRISPR-Cas9 is a key technology for targeted genome editing and regulation in a number of organisms including mammalian cells. It is a rapid, simple, and cost-effective solution. CRISPR-Cas system has recently gained much scientific and public attention. This volume covers CRISPR-Cas9 based mammalian genome editing, creating disease models, cancer therapy, neurological, heredity, blood disorders, defective gene correction, stem cells therapy, epigenetic modifications, patents, ethics, biosafety and regulatory issues challenges and opportunities. This book is a key source of information on mammalian genome editing available in a single volume. This book will be useful for beginners in mammalian genome editing and also students, researchers, scientists, policymakers, clinicians and stakeholders interested in genome editing in several areas. Offers basic understanding and a clear picture of mammalian genome editing through CRISPR-Cas systems Discusses how to create mammalian disease models, stem cell modification, epigenetic modifications, correction of defective gene in blood disorders, heredity, neurological disorders and many more Discusses the application of CRISPR-Cas9 systems in basic sciences, biomedicine, molecular biology, translational sciences, neurobiology, neurology, cancer, stem cells, and many more

Genome Editing and Engineering

Genome Editing and Engineering
Author: Krishnarao Appasani
Publsiher: Cambridge University Press
Total Pages: 135
Release: 2018-06-30
ISBN: 1316762084
Category: Science
Language: EN, FR, DE, ES & NL

Genome Editing and Engineering Book Excerpt:

Recent advances in genome editing tools using endonucleases such as TALENs, ZFNs, and CRISPRs, combined with genomic engineering technologies, have opened up a wide range of opportunities from applications in the basic sciences and disease biology research, to the potential for clinical applications and the development of new diagnostic tools. This complete guide to endonuclease-based genomic engineering gives readers a thorough understanding of this rapidly expanding field. Chapters cover the discovery, basic science, and application of these techniques, focusing particularly on their potential relevance to the treatment of cancer, and cardiovascular and immunological disease. The final section discusses the legal and ethical issues which accompany the technology. Providing authoritative coverage of the potential that genome editing and engineering have, this is an ideal reference for researchers and graduate students and those working in the biotechnology and pharmaceutical industries, as well as in a clinical setting.

Stem Cell and Gene Therapy for Cardiovascular Disease

Stem Cell and Gene Therapy for Cardiovascular Disease
Author: Emerson C. Perin,Leslie W. Miller,Doris Taylor,James T. Willerson
Publsiher: Academic Press
Total Pages: 538
Release: 2015-08-21
ISBN: 0128018631
Category: Science
Language: EN, FR, DE, ES & NL

Stem Cell and Gene Therapy for Cardiovascular Disease Book Excerpt:

Stem Cell and Gene Therapy for Cardiovascular Disease is a state-of-the-art reference that combines, in one place, the breadth and depth of information available on the topic. As stem cell and gene therapies are the most cutting-edge therapies currently available for patients with heart failure, each section of the book provides information on medical trials from contributors and specialists from around the world, including not only what has been completed, but also what is planned for future research and trials. Cardiology researchers, basic science clinicians, fellows, residents, students, and industry professionals will find this book an invaluable resource for further study on the topic. Provides information on stem and gene therapy medical trials from contributors and specialists around the world, including not only what has been completed, but also what is planned for future research and trials Presents topics that can be applied to allogeneic cells, mesenchymal cells, gene therapy, cardiomyoctyes, iPS cells, MAPC's, and organogenesis Covers the three areas with the greatest clinical trials to date: chronic limb ischemia, chronic angina, and acute MI Covers the prevailing opinions on how to harness the body’s natural repair mechanisms Ideal resource for cardiology researchers, basic science clinicians, fellows, residents, students, and industry professionals

Stem Cells and Cardiovascular Diseases

Stem Cells and Cardiovascular Diseases
Author: Shijun Hu,Lei Ye,Mingtao Zhao,Feng Lan
Publsiher: Frontiers Media SA
Total Pages: 135
Release: 2022-03-17
ISBN: 2889747212
Category: Science
Language: EN, FR, DE, ES & NL

Stem Cells and Cardiovascular Diseases Book Excerpt:

Genetics of Hypertension

Genetics of Hypertension
Author: Xi Cheng,Bina Joe
Publsiher: Biota Publishing
Total Pages: 56
Release: 2015-08-01
ISBN: 161504695X
Category: Science
Language: EN, FR, DE, ES & NL

Genetics of Hypertension Book Excerpt:

Hypertension, or elevated blood pressure, is a major risk factor for various cardiovascular, renal diseases, and stroke. The form of hypertension with no identifiable cause is referred to as Essential Hypertension. Familial studies indicate that Essential Hypertension is heritable and, thereby, classical genetic approaches have been applied on both human and other mammalian models of hypertension to map the locations of the allelic variants within quantitative trait loci for blood pressure. The post genome era has further elevated this area of research into large-scale genome-wide association studies of hypertension in humans. Collectively, these studies have resulted in the prioritization and cataloging of several genomic regions containing allelic variants as candidates linked or associated with essential hypertension. Further, they are providing evidence to suggest that the inheritance of hypertension is rather complex, encompassing multiple variants both within protein-coding and non-coding annotations, each of which may act independently or interactively with other genes and/or environmental factors to differentially regulate blood pressure. This book provides an overview of the various methods employed to study the genetics of hypertension and discuss the progress and prospects of this area of research that may contribute towards individualized clinical management of hypertension in the future.

Translational Cardiometabolic Genomic Medicine

Translational Cardiometabolic Genomic Medicine
Author: Annabelle Rodriguez-Oquendo
Publsiher: Academic Press
Total Pages: 352
Release: 2015-09-07
ISBN: 0128004746
Category: Science
Language: EN, FR, DE, ES & NL

Translational Cardiometabolic Genomic Medicine Book Excerpt:

Translational Cardiometabolic Genomic Medicine, edited by Dr. Annabelle Rodriguez-Oquendo, is an important resource to postgraduate (medical, dental and graduate) students, postdoctoral fellows, basic scientists, and physician scientists seeking to understand and expand their knowledge base in the field of genomic medicine as it is applied to cardiometabolic diseases. This handbook integrates cutting-edge experimental approaches such as chromatin immunoprecipitation paired end tagging (CHIA-PET), to population studies such as the Multi-Ethnic Study of Atherosclerosis. It encompasses a range of book chapters that highlight bioinformatic approaches to better understanding functionality of the noncoding regions of the human genome to the use of molecular diagnostic testing in predicting increased risk of cardiovascular diseases. Where applicable, this reference also includes chapters related to therapeutic options specifically aligned to molecular targets. Provides comprehensive research on translational genomic medicine Explains state-of-the-art genome editing for stem cells and mouse models with significant relevance to human cardiometabolic disease Includes discussions on the functional effects of single nucleotide polymorphisms and cardiometabolic diseases, stratified by sex and race Encompasses a range of book chapters that highlight bioinformatic approaches to better understanding functionality of the noncoding regions of the human genome

It s Complicated

It s Complicated
Author: Jeff Hsu
Publsiher: Unknown
Total Pages: 121
Release: 2013
ISBN: 1928374650XXX
Category: Electronic Book
Language: EN, FR, DE, ES & NL

It s Complicated Book Excerpt:

Coronary artery disease (CAD) and atrial fibrillation (AF) are two common complex diseases of the heart that both have strong genetic components. Although genome wide association studies in humans and genetic mapping studies in mouse models have identified genomic loci associated with these diseases, the functional genetic variants and the mechanisms by which they alter disease susceptibility are largely unknown. In this thesis we use mouse models and human atrial tissues to perform functional genomic studies to gain insight into these disease associated loci, with the central hypothesis that many disease associated variants function by regulating the expression of nearby genes. The major methods used in these studies are transcriptome profiling by expression microarrays and RNA sequencing followed by rigorous statistical analysis. Transcript expression can be considered as an intermediate phenotype to disease susceptibility; and, the identification of genetic variants that alter transcript expression may lead to the mechanism of disease association. In the first study we performed an intercross between AKR and DBA/2 inbred mouse lines to identify and replicate atherosclerosis modifier genes. Quantitative trait loci (QTL) for atherosclerosis on chromosome 2, 15, and 17 were replicated between studies. We quantified whole-genome expression of bone marrow derived macrophages and endothelial cells and found that much of the gene expression was under genetic control, including at the atherosclerosis QTLs, and that this was genetic regulation of gene expression was reproducible between experiments. In the second study we identified genes that are regulated differentially in macrophages from these two mouse strains upon cholesterol loading. We found that the atherosclerosis resistant strain had much higher up regulation of genes in the lysosomal pathway. Combining these two studies, we identified several atherosclerosis candidate modifier genes, such as \textit{Sys1}. In the third study we quantified the differences in gene expression between human left and right atria using RNA sequencing and found many genes differentially expressed at loci associated with AF in genome-wide association studies. In addition we identified a long non-coding RNA adjacent to \textit{PITX2}, at the locus with that has the highest association with AF. In the future these loci can be studied further using reporter assays to identify the causal variant as well as using genomic editing techniques to study the gene regulatory mechanism.

Bioengineering and Biotechnology Approaches in Cardiovascular Sciences

Bioengineering and Biotechnology Approaches in Cardiovascular Sciences
Author: Jianyi Zhang,Vahid Serpooshan,James J. H. Chong,Philippe Menasché
Publsiher: Frontiers Media SA
Total Pages: 135
Release: 2021-10-04
ISBN: 2889714624
Category: Science
Language: EN, FR, DE, ES & NL

Bioengineering and Biotechnology Approaches in Cardiovascular Sciences Book Excerpt:

Risk Factors for Cardiovascular Disease

Risk Factors for Cardiovascular Disease
Author: Johnny Chahine
Publsiher: BoD – Books on Demand
Total Pages: 228
Release: 2022-04-06
ISBN: 1839687088
Category: Medical
Language: EN, FR, DE, ES & NL

Risk Factors for Cardiovascular Disease Book Excerpt:

Risk Factors for Cardiovascular Disease raises awareness about the importance of early recognition and prevention of modifiable cardiovascular risk factors. Some non-modifiable factors, like diabetes, can even be impacted by lifestyle modification (like weight loss) early in the disease. This book also describes cardiovascular risk factors in different patient populations and work settings.

Genome Engineering via CRISPR Cas9 System

Genome Engineering via CRISPR Cas9 System
Author: Vijai Singh,Pawan K. Dhar
Publsiher: Academic Press
Total Pages: 384
Release: 2020-02-18
ISBN: 0128181419
Category: Science
Language: EN, FR, DE, ES & NL

Genome Engineering via CRISPR Cas9 System Book Excerpt:

Genome Engineering via CRISPR-Cas9 Systems presents a compilation of chapters from eminent scientists from across the globe who have established expertise in working with CRISPR-Cas9 systems. Currently, targeted genome engineering is a key technology for basic science, biomedical and industrial applications due to the relative simplicity to which they can be designed, used and applied. However, it is not easy to find relevant information gathered in a single source. The book contains a wide range of applications of CRISPR in research of bacteria, virus, algae, plant and mammalian and also discusses the modeling of drosophila, zebra fish and protozoan, among others. Other topics covered include diagnosis, sensor and therapeutic applications, as well as ethical and regulatory issues. This book is a valuable source not only for beginners in genome engineering, but also researchers, clinicians, stakeholders, policy makers, and practitioners interested in the potential of CRISPR-Cas9 in several fields. Provides basic understanding and a clear picture on how to design, use and implement the CRISPR-Cas9 system in different organisms Explains how to create an animal model for disease research and screening purposes using CRISPR Discusses the application of CRISPR-Cas9 systems in basic sciences, biomedicine, virology, bacteriology, molecular biology, neurology, cancer, industry, and many more

Genetic Causes of Cardiac Disease

Genetic Causes of Cardiac Disease
Author: Jeanette Erdmann,Alessandra Moretti
Publsiher: Springer Nature
Total Pages: 406
Release: 2019-11-27
ISBN: 3030273717
Category: Medical
Language: EN, FR, DE, ES & NL

Genetic Causes of Cardiac Disease Book Excerpt:

This book provides a comprehensive summary of the latest developments in the field of the genomics of cardiac disease. Written and edited by leading clinicians and scientists involved in the analysis and therapy of genetic cardiac disorders, it discusses the genetic causes of a variety of cardiac diseases, such as the complex genetics and etiology of congenital heart diseases. It also explores sex differences in prevalent diseases, genetics-based therapeutic strategies and the use of various animal models and alternatives. The book is intended for research scientists and clinical scientists in the cardiovascular field, human geneticists and cardiologists.

Metabolic Regulation in the Development of Cardiovascular Diseases

Metabolic Regulation in the Development of Cardiovascular Diseases
Author: Xiaoqiang Tang,Md. Shenuarin Bhuiyan,InKyeom Kim
Publsiher: Frontiers Media SA
Total Pages: 135
Release: 2021-12-06
ISBN: 2889718131
Category: Science
Language: EN, FR, DE, ES & NL

Metabolic Regulation in the Development of Cardiovascular Diseases Book Excerpt:

Cardiovascular Genetics and Genomics

Cardiovascular Genetics and Genomics
Author: Dhavendra Kumar,Perry Elliott
Publsiher: Springer
Total Pages: 920
Release: 2018-01-17
ISBN: 3319661140
Category: Medical
Language: EN, FR, DE, ES & NL

Cardiovascular Genetics and Genomics Book Excerpt:

This title reflects the exponential growth in the knowledge and information on this subject and defines the extensive clinical translation of cardiovascular genetics and genomics in clinical practice. This concise, clinically oriented text is targeted at a broad range of clinicians who manage patients and families with a wide range of heterogeneous inherited cardiovascular conditions. Cardiovascular Genetics and Genomics: Principles and Clinical Practice includes a concise and clear account on selected topics written by a team of leading experts on clinical cardiovascular genetics. Each chapter include key information to assist the clinician and case histories have been incorporated to reflect contemporary practice in clinical cardiovascular genetics and genomics. Therefore this will be of key importance to all professionals working in the discipline, from clinicians and trainees in cardiology, cardiac surgery, electrophysiology, immunology through geneticists, nursing staff and those involved in precision medicine.

From GWAS Hits to Treatment Targets

From GWAS Hits to Treatment Targets
Author: Jeanette Erdmann,Tanja Zeller
Publsiher: Frontiers Media SA
Total Pages: 135
Release: 2019-08-16
ISBN: 2889459829
Category: Electronic Book
Language: EN, FR, DE, ES & NL

From GWAS Hits to Treatment Targets Book Excerpt:

Genome-wide association (GWA) studies, as a prototype of large-scale OMICs studies, have advanced our understanding of the genetic basis of many common diseases. With respect to coronary artery disease (CAD) and cardiovascular risk factors, like lipids, blood pressure or BMI, they have identified hundreds of chromosomal loci that modulate disease risk. Despite their scientific success, GWA studies have been criticized for having failed so far in delivering diagnostically or therapeutically relevant products. However, the ability to achieve such goals has been strengthened recently by further layers of OMICs-based data, including large-scale transcriptomics data, and better annotation of regulatory sequences and epigenetic changes in the genome (e.g. through the ENCODE project), as well as novel tools for bioinformatics analysis, allowing a systems medicine based approach to be applied. All in all, the last decade with its “gold rush of genomic discovery” led to the identification of known and novel pathways involved in the pathogenesis of cardiovascular diseases and point to novel treatment targets. This Research Topic has gathered contributions from scientists working in the field of cardiovascular genetics who have common interests in understanding the pathomechanisms linking genetic association findings and disease to finally translate the findings from large-scale genetic studies into novel treatment options.

Braunwald s Heart Disease E Book

Braunwald s Heart Disease E Book
Author: Douglas P. Zipes,Peter Libby,Robert O. Bonow,Douglas L. Mann,Gordon F. Tomaselli
Publsiher: Elsevier Health Sciences
Total Pages: 2040
Release: 2018-01-09
ISBN: 0323555934
Category: Medical
Language: EN, FR, DE, ES & NL

Braunwald s Heart Disease E Book Book Excerpt:

Trusted by generations of cardiologists for the latest, most reliable guidance in the field, Braunwald’s Heart Disease, 11th Edition, remains your #1 source of information on rapidly changing clinical science, clinical and translational research, and evidence-based medicine. This award-winning text has been completely updated, providing a superior multimedia reference for every aspect of this fast-changing field, including new material about almost every topic in cardiology.