Chemical Modification Of Enzymes

Author by : Jaime Eyzaguirre
Languange : en
Publisher by : Ellis Horwood
Format Available : PDF, ePub, Mobi
Total Read : 99
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Chemical Modification Of Enzymes

Author by : J. EYZAGUIRRE (ed)
Languange : en
Publisher by : Unknown
Format Available : PDF, ePub, Mobi
Total Read : 93
Total Download : 402
File Size : 51,9 Mb
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Chemical Modification Of Enzymes

Author by : Boris Ivanovich Kurganov
Languange : en
Publisher by : Nova Science Pub Incorporated
Format Available : PDF, ePub, Mobi
Total Read : 22
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Chemical Modification Of Enzymes

Author by : Jaime Eyzaguirre
Languange : en
Publisher by : Ellis Horwood Limited
Format Available : PDF, ePub, Mobi
Total Read : 30
Total Download : 150
File Size : 53,5 Mb
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Development Of Novel Biological Nanomaterials Through Protein Chemical Modification

Author by : Bobbi S Stromer
Languange : en
Publisher by : Unknown
Format Available : PDF, ePub, Mobi
Total Read : 67
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File Size : 47,8 Mb
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Description : Enzymes are capable of catalyzing many different reactions, some of which are not possible through conventional organic chemistry methods. However, their sensitive nature to environmental conditions such as pH, temperature, solvents, and denaturants limits their applications. One method of stabilizing protein against denaturing conditions is to adsorb them onto the surface of nanomaterials such as 3D nanoparticles, 2D inorganic nanodisks or even creating carrier free protein nanoparticles with no support system present. However, even these methods have drawbacks of low enzyme loadings, loss in activity and cost of production. The object of this dissertation was to develop novel nanomaterials. First, to better understand the physical properties that effect protein adsorbing to nanoparticles, enzymes were modified chemically to alter net protein charge so that the relationship of charge on the enzyme to charge on â o-ZrP could be better understood. It was concluded that protein charge and ions present in solution play an important governing role in how much enzyme can be adsorbed onto a surface. Development of facile surface modification method for controlling the physical properties of nanoparticles for enhanced enzyme adsorption. Protein was modified chemically to have a net positive charge, resulting in favorable binding between the positively charge protein and negatively charge silica nanoparticle surface. Anionic catalytic enzymes were adsorbed onto this nanomaterial and their percent loading and catalytic activity assessed. For both enzymes investigated, percent loading (m/m) and catalytic activity retention were among the highest reported to date. Lastly a nanoparticle made of protein with independently tunable emission, size and surface properties was synthesized. These particles are biodegradable, nontoxic and capable of white emission. White emitting particles are highly sensitive to cellular local environment. The methods of investigation and interpretation of the results are discussed in full in this work.


Techniques In The Life Sciences

Author by : Anonim
Languange : en
Publisher by : Unknown
Format Available : PDF, ePub, Mobi
Total Read : 45
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Enzymes In Synthetic Organic Chemistry

Author by : Wong
Languange : en
Publisher by : Elsevier
Format Available : PDF, ePub, Mobi
Total Read : 63
Total Download : 275
File Size : 41,6 Mb
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Description : This book covers the most recent development of enzymatic organic synthesis, with particular focus on the use of isolated enzymes. It is organized into one introductory chapter dealing with the characteristics of enzymes as catalysts, and five chapters dealing with different types of chemical transformations. Methods for enzyme immobilization and stabilizaton, the use of enzymes in extreme environments, and the alteration of enzyme properties by chemical modification and site-directed mutagenesis for synthetic purposes are covered.


Chemical Reagents For Protein Modification Third Edition

Author by : Roger L. Lundblad
Languange : en
Publisher by : Springer Science & Business
Format Available : PDF, ePub, Mobi
Total Read : 90
Total Download : 611
File Size : 41,5 Mb
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Description : Revised and updated, Chemical Reagents for Protein Modification, Third Edition is an encyclopedic work describing the many approaches to the site-specific modification of proteins. More than 2,000 references are cited describing the development of the various reagents. This book explores how the use of site-specific protein modification relates to a wide variety of biotechnology processes and products, such as protein microarrays, hydrogels, controlled-release pharmaceuticals, and biotherapeutics, and proteomics. With chapters focusing on analyses of individual amino acids, additional chapters cover chemical cross-linking and the chemical cleavage of peptide chains. There is considerable reference to the use of site-specific chemical modification in proteomics. The author pays particular attention to the general environmental factors that influence the reactivity of individual amino acid residues. He presents techniques for the characterization of modified proteins, including mass spectrometry, and includes increased coverage of spectroscopic techniques and crystallographic analyses. This is followed by coverage of nitrosylation reactions and other non-enzymatic modification processes. Specific reference is given to the differential labeling of protein functional groups with reagents labeled with stable isotopes. Resources include a list of current journals that publish site-specific chemical modification studies, the most commonly used reagents and their properties, and a contact list of reagent suppliers. Chemical Reagents for Protein Modification, 3rd Edition presents the most frequently used methods for the site-specific chemical modification of proteins, techniques for protein characterization, precise laboratory data for factors that influence reactivity and reproducibility, and industry-specific resources.


Chemical Modification Of Lysozyme

Author by : Karl Joseph Kramer
Languange : en
Publisher by : Unknown
Format Available : PDF, ePub, Mobi
Total Read : 70
Total Download : 166
File Size : 42,8 Mb
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Chemical Reagents For Protein Modification

Author by : Roger L. Lundblad
Languange : en
Publisher by : CRC Press
Format Available : PDF, ePub, Mobi
Total Read : 31
Total Download : 802
File Size : 45,5 Mb
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Description : First published in 1991, Chemical Reagents for Protein Modification, 2nd Edition provides a unique combination of theoretical and practical considerations for the use of chemical reagents for site-specific modification of proteins. The book is divided into three sections, with the first section describing general techniques, including information on the organic chemistry of the various modification reactions; the separation and characterization of site-specific modified proteins, including applications to proteins separated by electrophoresis followed by blotting; the specific chemical cleavage of peptide bonds in proteins; the separation of peptides by high-performance liquid chromatography and electrophoresis; and the use of chemical reagents to assess conformational change in proteins. The second section provides an encyclopedic description of reagents and reactions for the site-specific modification of individual amino acid residues in proteins. The final section presents descriptions of the use of chemical reagents to label biologically significant sites in proteins, including enzyme active sites and the use of covalent cross-linking to measure protein-protein interactions. Particular emphasis is placed on the use of photoaffinity reagents. The book will be an extremely useful research tool for all investigators interested in the solution chemistry of proteins.


The Enzymes

Author by : Anonim
Languange : en
Publisher by : Academic Press
Format Available : PDF, ePub, Mobi
Total Read : 87
Total Download : 434
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Description : The Enzymes


Chemical Reagents For Protein Modification Fourth Edition

Author by : Roger L. Lundblad
Languange : en
Publisher by : CRC Press
Format Available : PDF, ePub, Mobi
Total Read : 16
Total Download : 254
File Size : 48,8 Mb
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Description : The use of the chemical modification of proteins has evolved over the past 80 years, benefiting from advances in analytical, physical, and organic chemistry. Over the past 30 years, the use of chemical reagents to modify proteins has been crucial in determining the function and structure of purified proteins. This groundbreaking work is part of the foundation of emerging disciplines of proteomics, chemical biology, structure biology, and chemical proteomics. Chemical Reagents for Protein Modification, Fourth Edition provides a comprehensive review of reagents used for the chemical modification of proteins, representing a major revision of the work presented in previous editions. The completely updated Fourth Edition is substantially larger and includes five new chapters: Alkylating Agents Acylating Agents Nitration and Nitrosylation Oxidation Modification of Proteins with Reducing Agents There is greatly increased coverage of the chemical modification of cysteine, which is critical for bioconjugate synthesis. The chapter on reduction also provides information necessary for bioconjugate synthesis as well as for the processing of inclusion bodies. The book places emphasis on conditions that affect the specificity of the chemical modification of proteins, such as solvent and temperature. The format has been markedly revised, presenting information based on the chemical nature of the modifying material and on the amino acid residue modified. This new version has increased significance to biopharmaceuticals. Much of the information is in tabular form, which enables the rapid location of cited material.


Chemical Modification Of Biological Polymers

Author by : Roger L. Lundblad
Languange : en
Publisher by : CRC Press
Format Available : PDF, ePub, Mobi
Total Read : 96
Total Download : 352
File Size : 42,6 Mb
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Description : Examining the chemical modification of biological polymers and the emerging applications of this technology, Chemical Modification of Biological Polymers reflects the change in emphasis in this subsection of biotechnology from the study of protein structure and function toward applications in therapeutics and diagnostics. Highlights The basic organic chemistry of the modification proteins, nucleic acids, oligosaccharides, polysaccharides, and their applications New analytical technologies used to characterize the chemical modification of biological polymers Identification of in vivo, non-enzymatic chemical modification of biological polymers Specific chemical modifications to generate biopharmaceutical products This book covers the basics on the organic chemistry underlying the chemical modification of biopolymers, including updates on the use of various chemical reagents. It describes the current status of chemical modification of biological polymers and emerging applications of this technology in biotechnology. These technologies are important for the manufacture of conjugate proteins used in drug delivery, for the preparation of nucleic acid microarrays, and for the preparation of hydrogels and other materials used in tissue engineering.


Chemical Reagents For Protein Modification

Author by : Roger L. Lundblad
Languange : en
Publisher by : CRC Press
Format Available : PDF, ePub, Mobi
Total Read : 17
Total Download : 320
File Size : 53,7 Mb
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Description : The contents of this book are focused on the use of chemical modification to study the properties of proteins in solution. Particular emphasis has been placed on the practical laboratory aspects of this approach to the study of the relationship between structure and function in the complex class of biological heteropolymers. As a result, little emphasis is given to the individual consideration of the functional consequence of chemical modification.


Phospholipase A2 From Cobra Venom

Author by : Paul Luther Darke
Languange : en
Publisher by : Unknown
Format Available : PDF, ePub, Mobi
Total Read : 72
Total Download : 997
File Size : 40,7 Mb
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Chemical Reagents For Protein Modification

Author by : Roger L. Lundblad
Languange : en
Publisher by : CRC PressI Llc
Format Available : PDF, ePub, Mobi
Total Read : 66
Total Download : 766
File Size : 48,6 Mb
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Description : Chemical Reagents for Protein Modification, 2nd Edition provides a unique combination of theoretical and practical considerations for the use of chemical reagents for site-specific modification of proteins. The book is divided into three sections, with the first section describing general techniques, including information on the organic chemistry of the various modification reactions; the separation and characterization of site-specific modified proteins, including applications to proteins separated by electrophoresis followed by blotting; the specific chemical cleavage of peptide bonds in proteins; the separation of peptides by high-performance liquid chromatography and electrophoresis; and the use of chemical reagents to assess conformational change in proteins. The second section provides an encyclopedic description of reagents and reactions for the site-specific modification of individual amino acid residues in proteins. The final section presents descriptions of the use of chemical reagents to label biologically significant sites in proteins, including enzyme active sites and the use of covalent cross-linking to measure protein-protein interactions. Particular emphasis is placed on the use of photoaffinity reagents. The book will be an extremely useful research tool for all investigators interested in the solution chemistry of proteins.