Techniques In The Life Sciences

Author by : Anonim
Languange : en
Publisher by : Unknown
Format Available : PDF, ePub, Mobi
Total Read : 26
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Molecular And Cellular Enzymology

Author by : Jeannine Yon-Kahn
Languange : en
Publisher by : Springer Science & Business Media
Format Available : PDF, ePub, Mobi
Total Read : 93
Total Download : 817
File Size : 54,9 Mb
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Description : Molecular and Cellular Enzymology addresses not only experienced enzymologists but also students, teachers and academic and industrial researchers who are confronted with enzymological problems during their fundamental or applied research. In this field there is an urgent need for training in order to meet the requirements of both research and industrial endeavours. This book consists of several levels. Practical aspects and elementary explanations are given for the benefit of non-specialists’ and students’ understanding. In order to facilitate the task of students, two typographies have been adopted. The main text corresponds to basic knowledge, whereas text in a smaller font provides more specialised information. Specialists will also find topics more deeply expounded with the principal bibliographic references cited. The bibliography, however, is not exhaustive; the choice includes general books and review articles as well as some specialised articles. In this book, for the first time, the different molecular and cellular aspects of enzymology are presented together. Until now, there has been no book available in which these different aspects are treated in the same volume. In addition, besides the theoretical developments, this book provides a wealth of practical information for experimentalists.


Chemical Reagents For Protein Modification Third Edition

Author by : Roger L. Lundblad
Languange : en
Publisher by : Springer Science & Business
Format Available : PDF, ePub, Mobi
Total Read : 76
Total Download : 876
File Size : 43,6 Mb
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Description : Revised and updated, Chemical Reagents for Protein Modification, Third Edition is an encyclopedic work describing the many approaches to the site-specific modification of proteins. More than 2,000 references are cited describing the development of the various reagents. This book explores how the use of site-specific protein modification relates to a wide variety of biotechnology processes and products, such as protein microarrays, hydrogels, controlled-release pharmaceuticals, and biotherapeutics, and proteomics. With chapters focusing on analyses of individual amino acids, additional chapters cover chemical cross-linking and the chemical cleavage of peptide chains. There is considerable reference to the use of site-specific chemical modification in proteomics. The author pays particular attention to the general environmental factors that influence the reactivity of individual amino acid residues. He presents techniques for the characterization of modified proteins, including mass spectrometry, and includes increased coverage of spectroscopic techniques and crystallographic analyses. This is followed by coverage of nitrosylation reactions and other non-enzymatic modification processes. Specific reference is given to the differential labeling of protein functional groups with reagents labeled with stable isotopes. Resources include a list of current journals that publish site-specific chemical modification studies, the most commonly used reagents and their properties, and a contact list of reagent suppliers. Chemical Reagents for Protein Modification, 3rd Edition presents the most frequently used methods for the site-specific chemical modification of proteins, techniques for protein characterization, precise laboratory data for factors that influence reactivity and reproducibility, and industry-specific resources.


Techniques In Protein Modification

Author by : Roger L. Lundblad
Languange : en
Publisher by : CRC Press
Format Available : PDF, ePub, Mobi
Total Read : 56
Total Download : 379
File Size : 49,7 Mb
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Description : This exciting laboratory support book encompasses the encyclopedic coverage of the most frequently used methods for the site-specific chemical modification of proteins. In a concise manner, it presents methods for the characterization of modified proteins, including: amino acid analysis protein sequence analysis chemical cleavage of protein chains chromatographic separation of peptides It also discusses various approaches to the determination of solution protein concentration. It includes a complete literature survey of the various reagents, a list of the most commonly used reagents with their physical and chemical properties, and a list of preferred reagent suppliers.


Enzymology Primer For Recombinant Dna Technology

Author by : Hyone-Myong Eun
Languange : en
Publisher by : Elsevier
Format Available : PDF, ePub, Mobi
Total Read : 44
Total Download : 187
File Size : 46,5 Mb
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Description : Enzymes are indispensable tools in recombinant DNA technology and genetic engineering. This book not only provides information for enzymologists, but does so in a manner that will also aid nonenymologists in making proper use of these biocatalysts in their research. The Enzymology Primer for Recombinant DNA Technology includes information not usually found in the brief descriptions given in most books on recombinant DNA methodology and gene cloning. Provides essential basics as well as up-to-date information on enzymes most commonly used in recombinant DNA technology Presents information in an easily accessible format to serve as a quick reference source Leads to a better understanding of the role of biocatalysts in recombinant DNA techniques


Current Catalog

Author by : National Library of Medicine (U.S.)
Languange : en
Publisher by : Unknown
Format Available : PDF, ePub, Mobi
Total Read : 14
Total Download : 929
File Size : 49,6 Mb
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Description : First multi-year cumulation covers six years: 1965-70.


Chemical Modification Of Enzymes

Author by : Jaime Eyzaguirre
Languange : en
Publisher by : Unknown
Format Available : PDF, ePub, Mobi
Total Read : 40
Total Download : 982
File Size : 52,5 Mb
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Chemical Reagents For Protein Modification

Author by : Roger L. Lundblad
Languange : en
Publisher by : CRC PressI Llc
Format Available : PDF, ePub, Mobi
Total Read : 69
Total Download : 648
File Size : 44,6 Mb
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Description : The chemical modification of proteins; Amino acid analysis; Peptide separation by reverse-phase high performance liquid chromatography; Methods for sequence determination; Chemical cleavage of peptide bonds; The modification of cystine; The Modification of cystine - cleavage of disulfide bonds; The modification of methionine; Modification of histidine residues; The modification of Lysine.


Bioanalytics

Author by : Friedrich Lottspeich
Languange : en
Publisher by : John Wiley & Sons
Format Available : PDF, ePub, Mobi
Total Read : 93
Total Download : 882
File Size : 40,5 Mb
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Description : Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids. The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a birdï¿1⁄2s eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists. The following techniques are covered in detail: - Purification and determination of proteins - Measuring enzymatic activity - Microcalorimetry - Immunoassays, affinity chromatography and other immunological methods - Cross-linking, cleavage, and chemical modification of proteins - Light microscopy, electron microscopy and atomic force microscopy - Chromatographic and electrophoretic techniques - Protein sequence and composition analysis - Mass spectrometry methods - Measuring protein-protein interactions - Biosensors - NMR and EPR of biomolecules - Electron microscopy and X-ray structure analysis - Carbohydrate and lipid analysis - Analysis of posttranslational modifications - Isolation and determination of nucleic acids - DNA hybridization techniques - Polymerase chain reaction techniques - DNA sequence and epigenetic modification analysis - Analysis of protein-nucleic acid interactions - Analysis of sequence data - Proteomics, metabolomics, peptidomics and toponomics - Chemical biology


Research In Photosynthesis

Author by : Norio Murata
Languange : en
Publisher by : Springer Science & Business Media
Format Available : PDF, ePub, Mobi
Total Read : 19
Total Download : 558
File Size : 53,7 Mb
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Chemistry And Biochemistry Of Flavoenzymes

Author by : Franz Muller
Languange : en
Publisher by : CRC Press
Format Available : PDF, ePub, Mobi
Total Read : 61
Total Download : 760
File Size : 50,9 Mb
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Description : Chemistry and Biochemistry of Flavoenzymes summarizes the present knowledge of the chemical and physical properties of free flavin, modified flavins occurring in nature, and deazaflavin. This information forms the fundamental basis for understanding the catalytic properties of flavoenzymes. Flavoproteins involved in transport, electron transfer, oxidation, dehydrogenation and hydroxylation reactions are discussed with respect to their biochemical and biophysical properties. The book presents the catalytic mechanisms of the flavoproteins in detail and, where available, three-dimensional structures and molecular biology data are included. The medical aspects of free and protein-bound flavin are also briefly discussed. Chemistry and Biochemistry of Flavoenzymes is an essential reference source for chemists, biochemists, toxicologists, biologists, pharmacologists, and researchers in the pharmaceutical industry.


Techniques In The Life Sciences

Author by : Anonim
Languange : en
Publisher by : Unknown
Format Available : PDF, ePub, Mobi
Total Read : 26
Total Download : 875
File Size : 43,5 Mb
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Description :


Selected Topics In The History Of Biochemistry Personal Recollections

Author by : G. Semenza
Languange : en
Publisher by : Elsevier
Format Available : PDF, ePub, Mobi
Total Read : 25
Total Download : 150
File Size : 49,8 Mb
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Description : As in Volumes 35 and 36, the chapters in this new volume complement, with personal recollections, the History of Biochemistry that was covered in the Comprehensive Biochemistry Series, Volumes 30-33 by M. Florkin and Volume 34A by P. Laszlo. The biographical and autobiographical chapters will convey to the reader a lively, albeit at times subjective, view of the scientific and social environment in which the authors have worked, resulting in new concepts and theories on the biological sciences.


Chemical Reagents For Protein Modification

Author by : Roger L. Lundblad
Languange : en
Publisher by : CRC Press
Format Available : PDF, ePub, Mobi
Total Read : 57
Total Download : 850
File Size : 48,6 Mb
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Description : The contents of this book are focused on the use of chemical modification to study the properties of proteins in solution. Particular emphasis has been placed on the practical laboratory aspects of this approach to the study of the relationship between structure and function in the complex class of biological heteropolymers.


Industrial Enzymes

Author by : Julio Polaina
Languange : en
Publisher by : Springer Science & Business Media
Format Available : PDF, ePub, Mobi
Total Read : 70
Total Download : 883
File Size : 55,6 Mb
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Description : Recent developments in genetic engineering and protein chemistry are bringing ever more powerful means of analysis to bear on the study of enzyme structure. This volume reviews the most important types of industrial enzymes. In a balanced manner it covers three interrelated aspects of paramount importance for enzyme performance: three-dimensional protein structure, physicochemical and catalytic properties, and the range of both classical and novel applications.