Engineering Optogentically Controlled Schwann Cells

Author by : Tasneem Hassan Muna
Languange : un
Publisher by :
Format Available : PDF, ePub, Mobi
Total Read : 47
Total Download : 351
File Size : 43,7 Mb
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Description : Spinal cord injury (SCI) affects millions of people worldwide. It is a complicated disease with no cure that leaves patients with permanent neurological deficits, mainly because damaged neurons fail to regenerate after SCI. Schwann cells (SC) play important role in supporting axon regeneration after peripheral nerve injury. Their regenerative potential makes them ideal candidates for use in SCI to support axon regeneration. However, a significant improvement in recovery is seen only when SC are combined with additional therapeutic agents. Administration of such agents often requires multiple injections, which increases chances of inflammation, infection and complicates dosage control. Engineered SC that produce therapeutic agents at the site of SCI can obviate the need for multiple injections. What is currently lacking is an approach that allows to control engineered SC post-transplantation. The objective of this project was to test an optogenetic approach to controlling gene expression in SC. Light in the near-infrared window (NIRW) encompasses the spectrum of 670 to 900 nm. NIRW light penetrates deeply through mammalian tissues and is safe for medical applications. NIRW light represents a non-invasive way of achieving spatio-temporal control over transplanted cells. In this study, we engineered SC stably expressing a NIRW light-responsive adenylyl cyclase, IlaM5. We showed that NIRW irradiation activates expression of a reporter luciferase from a cAMP-dependent promoter. Several fold increase in luciferase levels were observed following a one-hour long irradiation of SC. The activation levels were higher if biliverdin, the chromophore for IlaM5, was added to SC exogenously. This study represents a proof-of-concept that SC can be effectively engineered to respond to NIRW light, which opens opportunities for translational applications of engineered, NIRW light-controlled SC in spinal cord regeneration.


Optogenetics

Author by : Krishnarao Appasani
Languange : en
Publisher by : Cambridge University Press
Format Available : PDF, ePub, Mobi
Total Read : 65
Total Download : 729
File Size : 53,5 Mb
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Description : Discovered little more than a decade ago, optogenetics - a revolutionary technique combining genetic and optical methods to observe and control the function of neurons - is now a widely used research tool. Optogenetics-driven research has led to insights into Parkinson's disease and other neurological and psychiatric disorders. With contributions from leaders and innovators from both academia and industry, this volume explores the discovery and application of optogenetics, from the basic science to its potential clinical use. Chapters cover a range of optogenetics applications, including for brain circuits, plasticity, memory, learning, sleep, vision and neurodegenerative and neuropsychiatric diseases. Providing authoritative coverage of the huge potential that optogenetics research carries, this is an ideal resource for researchers and graduate students, as well as for those working in the biotechnology and pharmaceutical industries and in a clinical setting.


Big On Bk Current Insights Into The Function Of Large Conductance Voltage And Ca2 Activated K Channels At The Molecular Cellular And Systemic Levels

Author by :
Languange : en
Publisher by : Academic Press
Format Available : PDF, ePub, Mobi
Total Read : 59
Total Download : 239
File Size : 43,7 Mb
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Description : Big on Bk: Current Insights into the Function of Large Conductance Voltage- and Ca2+- Activated K+ Channels at the Molecular, Cellular and Systemic Levels, a volume in the International Review of Neurobiology series, is a comprehensive overview of the state-of-the-art research into this area. It reviews current knowledge and understanding, and also provides a starting point for researchers and practitioners entering the field. The latest volume in the International Review of Neurobiology series Provides a broad coverage of subject matter at the molecular, cellular and systemic levels Presents an ideal resource for researchers and practitioners, and those just entering the field


Animal Models For Medications Screening To Treat Addiction

Author by : Richard L Bell
Languange : en
Publisher by : Academic Press
Format Available : PDF, ePub, Mobi
Total Read : 11
Total Download : 393
File Size : 49,5 Mb
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Description : Animal Models for Medications Screening to Treat Addiction, the latest volume in the International Review of Neurobiology series, provides a comprehensive overview of the state-of-the-art research on the topic. It reviews the current knowledge and understanding in the field, presenting a starting point for researchers and practitioners entering the field. Brings together information on the current state of medication development for drug addiction using animal models Contains comprehensive descriptions of various models associated with many forms of drug addiction Adds new information for translational research in the field of drug addiction


Metal Related Neurodegenerative Disease

Author by :
Languange : en
Publisher by : Academic Press
Format Available : PDF, ePub, Mobi
Total Read : 31
Total Download : 313
File Size : 40,8 Mb
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Description : This issue reviews the role of metals in neurodegenerative diseases; including Parkinson’s and Huntington’s disease; restless leg syndrome and NBIA disorders; and Wilson’s disease and manganese and calcium accumulation disorders. An update on advances in neuroimaging and pathology of metal related disease is also presented. This volume of International Review of Neurobiology brings together cutting-edge research on metal related neurodegenerative disease It reviews the role of metals in neurodegenerative diseases, including Parkinson’s and Huntington’s disease; restless leg syndrome and NBIA disorders; and Wilson’s disease and manganese and calcium accumulation disorders An update on advances in neuroimaging and pathology of metal related disease is also presented


Neuroimmune Signaling In Drug Actions And Addictions

Author by :
Languange : en
Publisher by : Academic Press
Format Available : PDF, ePub, Mobi
Total Read : 31
Total Download : 678
File Size : 55,8 Mb
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Description : This well-established international series examines major areas of basic and clinical research within neuroscience, as well as emerging and promising subfields. This volume concentrates on Neuroimmune Signaling in Drug Actions and Addictions. This book looks at neuroimmune signaling in drug actions and addictions in the light of the newest scholarly discoveries and insights