Enzyme Kinetics And Mechanisms Part E Energetics Of Enzyme Catalysis

Author by : Anonim
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Publisher by : Elsevier
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Description : This volume supplements Volumes 63, 64, 87, and 249 of Methods in Enzymology. These volumes provide a basic source for the quantitative interpretation of enzyme rate data and the analysis of enzyme catalysis. Among the major topics covered are Engergetic Coupling in Enzymatic Reactions, Intermediates and Complexes in Catalysis, Detection and Properties of Low Barrier Hydrogen Bonds, Transition State Determination, and Inhibitors. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.


Enzyme Kinetics And Mechanism Part F Detection And Characterization Of Enzyme Reaction Intermediates

Author by : Daniel L. Purich
Languange : en
Publisher by : Elsevier
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Description : The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences. Spectroscopic Detection of Reaction Intermediates Isotopic and Kenetic Detection of Reaction Intermediates Chemical Trapping and Inhibitor Methods for Detecting Reaction Intermediates


Dna Replication

Author by : Judith L. Campbell
Languange : en
Publisher by : Academic Press
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Total Read : 86
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Description : The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Key Features * Includes descriptions of functional, structural, kinetic, and genetic methods for analyzing major enzymes of DNA replication * Describes strategies for studying interactions of these proteins during replication * Provides comprehensive descriptions of uses of prokaryotic and eukaryotic crude in vitro replication systems and reconstitution of such systems from purified proteins * Includes methods for analyzing DNA replication in vivo


Biothermodynamics

Author by : Michael L. Johnson
Languange : en
Publisher by : Academic Press
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Description : The use of thermodynamics in biological research can be equated to an energy book-keeping system. While the structure and function of a molecule is important, it is equally important to know what drives the energy force. This volume presents sophisticated methods for estimating the thermodynamic parameters of specific protein-protein, protein-DNA and small molecule interactions. * Elucidates the relationships between structure and energetics and their applications to molecular design, aiding researchers in the design of medically important molecules * Provides a "must-have" methods volume that keeps MIE buyers and online subscribers up-to-date with the latest research * Offers step-by-step lab instructions, including necessary equipment, from a global research community


Biothermodynamics

Author by : Anonim
Languange : en
Publisher by : Academic Press
Format Available : PDF, ePub, Mobi
Total Read : 66
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Description : The use of thermodynamics in biological research can be equated to an energy book-keeping system. While the structure and function of a molecule is important, it is equally important to know what drives the energy force. This volume presents sophisticated methods for estimating the thermodynamic parameters of specific protein-protein, protein-DNA and small molecule interactions. * Elucidates the relationships between structure and energetics and their applications to molecular design, aiding researchers in the design of medically important molecules * Provides a "must-have" methods volume that keeps MIE buyers and online subscribers up-to-date with the latest research * Offers step-by-step lab instructions, including necessary equipment, from a global research community


Liposomes

Author by : Anonim
Languange : en
Publisher by : Elsevier
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Description : Liposomes are cellular structures made up of lipid molecules. Important as a cellular model in the study of basic biology, liposomes are also used in clinical applications such as drug delivery and virus studies. Liposomes Part D is a continuation of previous Methods in Enzymology Liposome volumes A, B, and C. Covers antibody or ligand targeted liposomes; environment sensitive liposomes; liposomal oligonucleotides; liposomes in vivo


Enzyme Kinetics

Author by : Alejandro G. Marangoni
Languange : en
Publisher by : John Wiley & Sons
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Total Read : 75
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Description : Practical Enzyme Kinetics provides a practical how-to guide for beginning students, technicians, and non-specialists for evaluating enzyme kinetics using common software packages to perform easy enzymatic analyses.


Macromolecular Crystallography Part D

Author by : Charles W. Carter, Jr.
Languange : en
Publisher by : Gulf Professional Publishing
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Total Read : 88
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Description : Accurate molecular structures is vital for rational drug design and for structure based functional studies directed toward the development of effective therapeutic agents and drugs. Crystallography can reliably predict structure, both in terms of folding and atomic details of bonding. * Phases * Map interpretation and refinement * Analysis and software


Numerical Computer Methods

Author by : Anonim
Languange : en
Publisher by : Elsevier
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Description : The aim of Numerical Computer Methods, Part D is to brief researchers of the importance of data analysis in enzymology, and of the modern methods that have developed concomitantly with computer hardware. It is also to validate researchers' computer programs with real and synthetic data to ascertain that the results produced are what they expected. Selected Contents: Prediction of protein structure Modeling and studying proteins with molecular dynamics Statistical error in isothermal titration calorimetry Analysis of circular dichroism data Model comparison methods


Energetics Of Biological Macromolecules

Author by : Jo M. Holt
Languange : en
Publisher by : Elsevier
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Description : This volume focuses on the cooperative binding aspects of energetics in biological macromolecules. Methodologies such as NMR, small-angle scattering techniques for analysis, calorimetric analysis, fluorescence quenching, and time resolved FRET measurements are discussed. *Methods for Evaluating Cooperativity in a Dimeric Hemoglobin *Multiple-Binding of Ligands to a Linear Biopolymer *Fluorescence Quenching Methods to Study Protein-Nucleic Acid Interactions *Linked Equilibria in Biotin Repressor Function: Thermodynamic, Structural and Kinetic Analysis


Enzyme Kinetics Catalysis And Control

Author by : Daniel L. Purich
Languange : en
Publisher by : Elsevier
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Total Read : 14
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Description : Far more than a comprehensive treatise on initial-rate and fast-reaction kinetics, this one-of-a-kind desk reference places enzyme science in the fuller context of the organic, inorganic, and physical chemical processes occurring within enzyme active sites. Drawing on 2600 references, Enzyme Kinetics: Catalysis & Control develops all the kinetic tools needed to define enzyme catalysis, spanning the entire spectrum (from the basics of chemical kinetics and practical advice on rate measurement, to the very latest work on single-molecule kinetics and mechanoenzyme force generation), while also focusing on the persuasive power of kinetic isotope effects, the design of high-potency drugs, and the behavior of regulatory enzymes. Historical analysis of kinetic principles including advanced enzyme science Provides both theoretical and practical measurements tools Coverage of single molecular kinetics Examination of force generation mechanisms Discussion of organic and inorganic enzyme reactions


Rna Polymerase And Associated Factors

Author by : Sankar Adhya
Languange : en
Publisher by : Elsevier
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Total Read : 70
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Description : RNA polymerase is molecule important to gene transcription. Along with associated factors, RNA polymerase is part of the process in which RNA is transcribed to produce a protein. * Models and methods for studying polymerase translocation * Assay for movements of RNA polymerase along DNA * Engineering of elongation complexes of bacterial and yeast RNA polymerases


Nitric Oxide

Author by : Anonim
Languange : en
Publisher by : Elsevier
Format Available : PDF, ePub, Mobi
Total Read : 22
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Description : The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences. Presents information on nitrosothiols and nitric oxide in cell signaling Details nitric oxide and mitochondrial functions Explains nitric oxide synthases


Regulators And Effectors Of Small Gtpases Part D Rho Family

Author by : Anonim
Languange : en
Publisher by : Elsevier
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Description : This volume and its companions (Volumes 255, 256, 257, and the forthcoming 329) cover all biochemical and biological assays currently in use for analyzing the role of small GTPases in these aspects of cell biology at the molecular level. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.


Quinones And Quinone Enzymes

Author by : Helmut Sies
Languange : en
Publisher by : Elsevier
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Description : Quinones are members of a class of aromatic compounds with two oxygen atoms bonded to the ring as carbonyl groups. This volume covers the role of quinines enzymes in cellular signalling and modulation of gene expression. *Coenzyme Q: Detection and Quinone Reductases *Plasma Membrane Quinone Reductases *Quinones, Cellular Signaling, and Modulation of Gene Expression


Chromatin And Chromatin Remodeling Enzymes

Author by : Carl Wu
Languange : en
Publisher by : Elsevier
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Total Read : 91
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Description : DNA in the nucleus of plant and animal cells is stored in the form of chromatin. Chromatin and the chromatin remodelling enzymes play an important role in gene transcription. Genetic assays of chromatin modification and remodeling Histone modifying enzymes ATP-dependent chromatin remodeling enzymes


Chromatin And Chromatin Remodeling Enzymes

Author by : Carl Wu
Languange : en
Publisher by : Elsevier
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Total Read : 69
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Description : DNA in the nucleus of plant and animal cells is stored in the form of chromatin. Chromatin and the Chromatin remodelling enzymes play an important role in gene transcription.


Chromatin And Chromatin Remodeling Enzymes

Author by : Carl Wu
Languange : en
Publisher by : Elsevier
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Total Read : 18
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Description : DNA in the nucleus of plant and animal cells is stored in the form of chromatin. Chromatin and the Chromatin remodellng enzymes play an important role in gene transcription. *Histone Bioinformatics *Biochemistry of histones, nucleosomes and chromatin *Molecular cytology of chromatin functions


Complex Enzymes In Microbial Natural Product Biosynthesis Part A Overview Articles And Peptides

Author by : Anonim
Languange : en
Publisher by : Academic Press
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Total Read : 40
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Description : Microbial natural products have been an important traditional source of valuable antibiotics and other drugs but interest in them waned in the 1990s when big pharma decided that their discovery was no longer cost-effective and concentrated instead on synthetic chemistry as a source of novel compounds, often with disappointing results. Moreover understanding the biosynthesis of complex natural products was frustratingly difficult. With the development of molecular genetic methods to isolate and manipulate the complex microbial enzymes that make natural products, unexpected chemistry has been revealed and interest in the compounds has again flowered. This two-volume treatment of the subject will showcase the most important chemical classes of complex natural products: the peptides, made by the assembly of short chains of amino acid subunits, and the polyketides, assembled from the joining of small carboxylic acids such as acetate and malonate. In both classes, variation in sub-unit structure, number and chemical modification leads to an almost infinite variety of final structures, accounting for the huge importance of the compounds in nature and medicine. * Gathers tried and tested methods and techniques from top players in the field. * In depth coverage of ribosomally-synthesised and Non-ribosomally-synthesised peptides. * Provides an extremely useful reference for the experienced research scientist.


Complex Enzymes In Microbial Natural Product Biosynthesis Part B Polyketides Aminocoumarins And Carbohydrates

Author by : Anonim
Languange : en
Publisher by : Academic Press
Format Available : PDF, ePub, Mobi
Total Read : 11
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Description : Microbial natural products have been an important traditional source of valuable antibiotics and other drugs but interest in them waned in the 1990s when big pharma decided that their discovery was no longer cost-effective and concentrated instead on synthetic chemistry as a source of novel compounds, often with disappointing results. Moreover understanding the biosynthesis of complex natural products was frustratingly difficult. With the development of molecular genetic methods to isolate and manipulate the complex microbial enzymes that make natural products, unexpected chemistry has been revealed and interest in the compounds has again flowered. This two-volume treatment of the subject will showcase the most important chemical classes of complex natural products: the peptides, made by the assembly of short chains of amino acid subunits, and the polyketides, assembled from the joining of small carboxylic acids such as acetate and malonate. In both classes, variation in sub-unit structure, number and chemical modification leads to an almost infinite variety of final structures, accounting for the huge importance of the compounds in nature and medicine. * Gathers tried and tested methods and techniques from top players in the field. * Provides an extremely useful reference for the experienced research scientist. * Covers biosynthesis of Polyketides, Tarpenoids, Aminocoumarins and Crabohydrates


Guide To Techniques In Mouse Development Part B

Author by : Paul Wassarman
Languange : en
Publisher by : Academic Press
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Total Read : 35
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Description : This volume comprehensively covers new technologies and methodologies that have appeared for the study of mouse development. This volume is Part B of an update of volume 225, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993. Comprehensively covers new techniques for the cryopreservation of gametes and embryos, production of transgenic and null (knockout) animals (use of ES cells), generation of conditional/inducible mutant animals, use of gene-trap mutagenesis, analysis of allele-specific expression, use of new reporter constructs, humanizing of transgenic animals, transcript profiling of mouse development, imaging of mouse development, and rederivation of animals and use of mouse genomics.


Guide To Techniques In Mouse Development Part A

Author by : Anonim
Languange : en
Publisher by : Academic Press
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Total Read : 65
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Description : This volume comprehensively covers new technologies and methodologies that have appeared for the study of mouse development. This volume is an update of volume 225 of MIE, "Guide to Techniques in Mouse Development", edited by P.M. Wassarman and M.L. DePamphilis and published in 1993. During the past 17 years many new technologies or methodologies have appeared for the study of mouse development and this volume comprehensively covers these, including: new techniques for the cryopreservation of gametes and embryos, production of transgenic and null (knockout) animals (use of ES cells), generation of conditional/inducible mutant animals, use of gene-trap mutagenesis, analysis of allele-specific expresion, use of new reporter constructs, humanizing of transgenic animals, transcript profiling of mouse development, imaging of mouse development, rederivation of animals and use of mouse genomics.


Hyperthermophilic Enzymes

Author by : Michael W. W. Adams
Languange : en
Publisher by : Academic Press
Format Available : PDF, ePub, Mobi
Total Read : 67
Total Download : 220
File Size : 41,9 Mb
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Description : The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences. This volume and its companions (Volumes 330 and 331) cover all current knowledge concerning hyperthermophilic enzymes. Major topics in this volume include redox and thiol-dependent proteins, nucleic acid modifying enzymes, and protein stability from biochemical and biophysical standpoints.


Hyperthermophilic Enzymes

Author by : Anonim
Languange : en
Publisher by : Elsevier
Format Available : PDF, ePub, Mobi
Total Read : 64
Total Download : 492
File Size : 52,5 Mb
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Description : The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences. This volume and its companions (Volumes 330 and 331) cover all current knowledge concerning hyperthermophilic enzymes. Major topics in this volume include redox and thiol-dependent proteins, nucleic acid modifying enzymes, and protein stability from biochemical and biophysical standpoints.


Hypertheromphilic Enzymes

Author by : Anonim
Languange : en
Publisher by : Elsevier
Format Available : PDF, ePub, Mobi
Total Read : 17
Total Download : 440
File Size : 55,9 Mb
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Description : The critically acclaimed laboratory standard for more than forty years, Methods in Enzymologyis one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. This volume and its companions (Volumes 31 and 334) cover all current knowledge concerning hyperthermophilic enzymes. Major topics in this volume include enzyme discovery, saccharolytic enzymes, and proteolytic enzymes.


Quinones And Quinone Enzymes

Author by : Anonim
Languange : en
Publisher by : Elsevier
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Total Read : 14
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Description : Quinones are members of a class of aromatic compounds with two oxygen atoms bonded to the ring as carbonyl groups. This volume covers more clinical aspects of quinines, such as anticancer properties, as well as their role in nutrition and in age-related diseases. Mitochondrial Ubiquinone and Reductases Anticancer Quinones and Quinone Oxido-Reductases Quininone Reductases: Chemoprevention, Nutrition Quinones and Age-Related Diseases


Protein Sensors And Reactive Oxygen Species

Author by : Helmut Sies
Languange : en
Publisher by : Academic Press
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Total Read : 59
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Description : This volume of Methods in Enzymology is a companion to Volume 347 and addresses direct sensing of reactive oxygen species and related free radicals by thiol enzymes and proteins.