Description : Synthetic Biology: A Lab Manual is the first manual for laboratory work in the new and rapidly expanding field of synthetic biology. Aimed at non-specialists, it details protocols central to synthetic biology in both education and research. In addition, it provides all the information that teachers and students from high schools and tertiary institutions need for a colorful lab course in bacterial synthetic biology using chromoproteins and designer antisense RNAs. As a bonus, practical material is provided for students of the annual international Genetically Engineered Machine (iGEM) competition. The manual is based upon a highly successful course at Sweden's Uppsala University and is coauthored by one of the pioneers of synthetic biology and two bioengineering postgraduate students.An inspiring foreword is written by another pioneer in the field, Harvard's George Church: “Synthetic biology is to early recombinant DNA as a genome is to a gene. Is there anything that SynBio will not impact? There was no doubt that the field of SynBio needed ‘A Lab Manual’ such as the one that you now hold in your hands.”
Description : This laboratory guide, intended for undergraduate and postgraduate students, includes techniques and their protocols ranging from microscopy to in vitro protein synthesis. Experiments relating to chromosomes study and identifying the phases of cell division are explained. The book lucidly deals with the extraction and characteri-zation of chromatin and techniques for studying its modifications, the gene methodology for identification of mutation and the methodology for isolation of nucleic acids from all types of organisms, such as viruses, fungi, plants and animals. All the protocols have been explained following step-by-step method. Different types of electrophoresis and their techniques, including blotting techniques and the methodology for stripping of probes from membranes for reusing the blot, have also been dealt with. Protocols on modern molecular biology techniques—PCR, restriction enzyme digest, DNA isolation, cloning and DNA sequencing—add weightage to the book. It also gives necessary knowledge of different types of stains, staining techniques, buffers, reagents and media used in the protocols. To help students prepare for answering viva voce questions, the book includes MCQs based on the discussed techniques.
Description : THE MADER/WINDELSPECHT STORY... The twelfth edition of Biology is a traditional, comprehensive introductory biology textbook, with coverage from Cell Structure and Function to the Conservation of Biodiversity. The book, which centers on the evolution and diversity of organisms, is appropriate for any one- or two-semester biology course. Biology, 12th Edition is the epitome of Sylvia Mader's expertise. Its concise, precise writing-style employs lucid language to present the material as succinctly as possible, enabling students—even non-majors—to master the foundational concepts before coming to class. “Before You Begin”, “Following the Themes”, and “Thematic Feature Readings” piece together the three major themes of the text—evolution, nature of science, and biological systems. Students are consistently engaged in these themes, revealing the interconnectedness of the major topics in biology. Sylvia Mader typifies an icon of science education. Her dedication to her students, coupled with her clear, concise writing-style has benefited the education of thousands of students over the past three decades. The integration of the text and digital world has been achieved with the addition of Dr. Michael Windelspecht’s facility for the development of digital learning assets. For over ten years, Michael served as the Introductory Biology Coordinator at Appalachian State University—a program that enrolls over 4,500 non-science majors annually. Michael is the lead architect in the design of McGraw-Hill's Connect Plus and LearnSmart media content for the Mader series. These assets allow instructors to easily design interactive tutorial materials, enhance presentations in both online and traditional environments, and assess the learning objectives and outcomes of the course.