RNA Methodologies

RNA Methodologies
Author: Robert E. Farrell, Jr.
Publsiher: Academic Press
Total Pages: 744
Release: 2009-08-31
ISBN: 9780080884950
Category: Science
Language: EN, FR, DE, ES & NL

RNA Methodologies Book Excerpt:

This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling, including quantification issues and elucidation of alternative transcription start sites. Collectively the chapters work together providing analysis with clear take-home lessons to assist researchers to understand RNA and to optimize time at the bench. The abundant use of flow charts, tables and graphs are especially helpful in the planning and implementation phases of a project and facilitate learning. 30% new material in this edition includes the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary. * 30% new material with the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques useful for all labs

RNA Methodologies

RNA Methodologies
Author: Robert E. Farrell
Publsiher: Academic Press
Total Pages: 767
Release: 2005
ISBN: 0122496965
Category: Science
Language: EN, FR, DE, ES & NL

RNA Methodologies Book Excerpt:

This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5' and 3' RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis. * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects

RNA Methodologies

RNA Methodologies
Author: Gerard Meurant
Publsiher: Academic Press
Total Pages: 317
Release: 2012-12-02
ISBN: 0323137792
Category: Science
Language: EN, FR, DE, ES & NL

RNA Methodologies Book Excerpt:

This book is a collection of tried and tested laboratory protocols for the isolation and characterization of mammalian RNA. It studies cellular regulation using RNA as a parameter of gene express, offers RNA isolation strategies, and explains proper handling, storage, and manipulation of RNA. * Studies cellular regulation using RNA as a parameter of Gene Expression * Offers RNA isolation strategies * Explains proper handling, storage, and manipulation of RNA

RNA Methodologies

RNA Methodologies
Author: Robert E. Farrell, Jr.
Publsiher: Elsevier
Total Pages: 688
Release: 2010-07-22
ISBN: 0080454763
Category: Science
Language: EN, FR, DE, ES & NL

RNA Methodologies Book Excerpt:

This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5’ and 3’ RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis. * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects

RNA Methodologies

RNA Methodologies
Author: Robert E. Farrell, Jr.
Publsiher: Academic Press
Total Pages: 876
Release: 2017-08-11
ISBN: 0128046791
Category: Science
Language: EN, FR, DE, ES & NL

RNA Methodologies Book Excerpt:

RNA Methodologies, Fifth Edition continues its tradition of excellence in providing the most up-to-date ribonucleic acid lab techniques for seasoned scientists and graduate students alike. This edition features new material on the exploding field of microRNA as well as the methods for the profiling of gene expression, both which have changed considerably in recent years. As a leader in the field, Dr. Farrell provides a wealth of knowledge on the topic of RNA while also giving readers helpful hints from his own personal experience in this subject area. Beginning with the most contemporary, RNA Methodologies, Fifth Edition, presents the essential techniques to use when working with RNA for the experienced practitioner while at the same time providing images and examples to aid the beginner in fully understanding this important branch of molecular biology. The next generation of scientists can look to this work as a guide for ensuring high productivity and highly representative data, as well as best practices in troubleshooting laboratory problems when they arise. Features new material in miRNA, MIQE guidelines, biomarkers, RNA sequencing, digital PCR and more Includes expanded coverage on quantitative PCR techniques, RNAi, bioinformatics, the role of locked nucleic acids, aptamer biology, PCR arrays, and other modern technologies Presents comprehensive, cutting-edge information covering all aspects of working with RNA Builds from basic information on RNA techniques to in-depth protocols to guidance on how to modify and adjust each step of a particular application Presents multiple avenues for addressing the same experimental goals

RNA Methodologies

RNA Methodologies
Author: Robert E. Farrell
Publsiher: Academic Press
Total Pages: 717
Release: 2010
ISBN: 9780123747273
Category: Science
Language: EN, FR, DE, ES & NL

RNA Methodologies Book Excerpt:

This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling, including quantification issues and elucidation of alternative transcription start sites. Collectively the chapters work together providing analysis with clear take-home lessons to assist researchers to understand RNA and to optimize time at the bench. The abundant use of flow charts, tables and graphs are especially helpful in the planning and implementation phases of a project and facilitate learning. 30% new material in this edition includes the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary. * 30% new material with the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques useful for all labs

Flow Cytometry

Flow Cytometry
Author: Anonim
Publsiher: Elsevier
Total Pages: 591
Release: 1994-12-12
ISBN: 0080859399
Category: Science
Language: EN, FR, DE, ES & NL

Flow Cytometry Book Excerpt:

Flow Cytometry, Second Edition provides a complete and comprehensive two volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who must use flow cytometry to separate and analyze cells. Key Features * Completely revised and greatly expanded since the publication of the First Edition in 1990 * Methods cover cell death and cell cycle analyses Practical, handbook-style presentation works in lab or classroom * Unique comprehensive methodological coverage * Color plates illustrate techniques * In-depth treatment of procedures, including a description of each procedure: * Theoretical foundations * Critical aspects * Possible pitfalls * Written by authors with extensive experience who: * Developed or modified the techniques * Describe their experience with different instruments and applications to different cell systems * Are the Who's Who in Flow Cytometry

RNA Methodologies

RNA Methodologies
Author: Robert E. Farrell, Jr.
Publsiher: Academic Press
Total Pages: 920
Release: 2022-10-15
ISBN: 9780323902212
Category: Science
Language: EN, FR, DE, ES & NL

RNA Methodologies Book Excerpt:

RNA Methodologies: A Laboratory Guide for Isolation and Characterization, Sixth Edition provides the most up-to-date ribonucleic acid lab techniques for seasoned scientists and graduate students alike. This edition features new material on RNA sequencing, RNA in Situ Hybridization, non-coding RNAs, computational RNA biology, transcriptomes and bioinformatics, along with the latest advances in methods and protocols across the field of RNA investigation. As a leader in the field, Dr. Farrell provides a wealth of knowledge on the topic of RNA biology while also giving readers helpful hints and troubleshooting techniques from his own personal experience in this subject area. This book presents the essential knowledge and techniques to use when working with RNA for the experienced practitioner, while also aiding the beginner in fully understanding this important branch of molecular biology. Presents the latest information covering all aspects of working with RNA, delivering a holistic understanding of this leading field in molecular biology Builds from basic information on RNA techniques to in-depth protocols for specific applications Features new chapters on RNA sequencing and RNA in situ hybridization Includes new material on RNA clinical applications and innovations, including RNA therapeutics and RNA vaccines, with particular relevance to coronavirus Comprises the latest developments in transcriptomes and bioinformatics, with new material on computational RNA biology, RNA CHiP analysis, aptamer biology and RNA epigenetics

RNA ligand Interactions Structural biology methods Semisynthetic methodologies RNA structure determination Techniques for monitoring RNA conformation and dynamics Modeling tertiary structure

RNA ligand Interactions  Structural biology methods  Semisynthetic methodologies   RNA structure determination   Techniques for monitoring RNA conformation and dynamics   Modeling tertiary structure
Author: Daniel Walter Celander,John Abelson
Publsiher: Unknown
Total Pages: 135
Release: 2000
ISBN: 1928374650XXX
Category: Ligands (Biochemistry)
Language: EN, FR, DE, ES & NL

RNA ligand Interactions Structural biology methods Semisynthetic methodologies RNA structure determination Techniques for monitoring RNA conformation and dynamics Modeling tertiary structure Book Excerpt:

Adrenergic Receptor Protocols

Adrenergic Receptor Protocols
Author: Curtis A. Machida
Publsiher: Springer Science & Business Media
Total Pages: 577
Release: 2000
ISBN: 1592596843
Category: Medical
Language: EN, FR, DE, ES & NL

Adrenergic Receptor Protocols Book Excerpt:

In Adrenegic Receptor Protocols, Curtis Machida and a panel of expert investigators present a comprehensive collection of modern molecular methods for analyzing adrenergic receptors and corresponding second messenger systems. These proven and readily reproducible techniques utilize genetic, RNA, protein expression, transactivator, and second messenger methodologies, as well as immunocytochemical, electrophysiological, transgenic, and in situ hybridization approaches. Each of the experts writing here details the use of their chosen method in examining the adrenergic receptor system, using aspects of the genetic flow of information as a guide: DNA ' RNA ' transactivator ' protein expression ' second messenger analyses ' cellular analyses ' transgenic whole animal approaches. Comprehensive and rich in practical detail, Adrenergic Receptor Protocols provides the first collection of reproducible methods for the study of these important regulators of CNS-mediated behavior and neural function. Its state-of-the-art methods constitute today's gold standard reference for all neurobiologists, neurochemists, neurologists, and pharmacologists studying this exceptionally important class of receptors.

Functional Genomics and Proteomics in the Clinical Neurosciences

Functional Genomics and Proteomics in the Clinical Neurosciences
Author: Scott E. Hemby,Sabine Bahn
Publsiher: Elsevier
Total Pages: 402
Release: 2006-10-09
ISBN: 9780080465630
Category: Science
Language: EN, FR, DE, ES & NL

Functional Genomics and Proteomics in the Clinical Neurosciences Book Excerpt:

The purpose of this work is to familiarize neuroscientists with the available tools for proteome research and their relative abilities and limitations. To know the identities of the thousands of different proteins in a cell, and the modifications to these proteins, along with how the amounts of both of these change in different conditions would revolutionize biology and medicine. While important strides are being made towards achieving the goal of global mRNA analysis, mRNA is not the functional endpoint of gene expression and mRNA expression may not directly equate with protein expression. There are many potential applications for proteomics in neuroscience: determination of the neuro-proteome, comparative protein expression profiling, post-translational protein modification profiling and mapping protein-protein interactions, to name but a few. Functional Genomics and Proteomics in Clinical Neuroscience will comment on all of these applications, but with an emphasis on protein expression profiling. This book combines the basic methodology of genomics and proteomics with the current applications of such technologies in understanding psychiatric illnesses. * Introduction of basic methodologies in genomics and proteomics and their integration in psychiatry * Development of the text in sections related to methods, application and future directions of these rapidly advancing technologies * Use of actual data to illustrate many principles of functional genomics and proteomics. * Introduction to bioinformatics and database management techniques

HIV Testing

HIV Testing
Author: Ricardo Diaz
Publsiher: BoD – Books on Demand
Total Pages: 146
Release: 2012-01-18
ISBN: 9533078715
Category: Medical
Language: EN, FR, DE, ES & NL

HIV Testing Book Excerpt:

It can be said that now is the best time for everyone infected to become aware of their own HIV status. The state of the art in HIV management progressively reveals that antiretroviral treatment can prevent transmission, as well as chronic damage in the human body, if started early. Unfortunately, antiretrovirals are not widely available in many places, especially in developing countries. In these parts of the world, diagnosis of HIV infection must be kept in the agenda as a priority, in order to understand specific details of local epidemics and as an effort to interrupt the chain of HIV transmission.

Using the Biological Literature

Using the Biological Literature
Author: Diane Schmidt
Publsiher: CRC Press
Total Pages: 412
Release: 2014-04-14
ISBN: 1466558571
Category: Language Arts & Disciplines
Language: EN, FR, DE, ES & NL

Using the Biological Literature Book Excerpt:

The biological sciences cover a broad array of literature types, from younger fields like molecular biology with its reliance on recent journal articles, genomic databases, and protocol manuals to classic fields such as taxonomy with its scattered literature found in monographs and journals from the past three centuries. Using the Biological Literature: A Practical Guide, Fourth Edition is an annotated guide to selected resources in the biological sciences, presenting a wide-ranging list of important sources. This completely revised edition contains numerous new resources and descriptions of all entries including textbooks. The guide emphasizes current materials in the English language and includes retrospective references for historical perspective and to provide access to the taxonomic literature. It covers both print and electronic resources including monographs, journals, databases, indexes and abstracting tools, websites, and associations—providing users with listings of authoritative informational resources of both classical and recently published works. With chapters devoted to each of the main fields in the basic biological sciences, this book offers a guide to the best and most up-to-date resources in biology. It is appropriate for anyone interested in searching the biological literature, from undergraduate students to faculty, researchers, and librarians. The guide includes a supplementary website dedicated to keeping URLs of electronic and web-based resources up to date, a popular feature continued from the third edition.

The Polymerase Chain Reaction

The Polymerase Chain Reaction
Author: Kary B. Mullis,Francois Ferre,Richard A. Gibbs
Publsiher: Springer Science & Business Media
Total Pages: 458
Release: 2012-02-02
ISBN: 1461202574
Category: Medical
Language: EN, FR, DE, ES & NL

The Polymerase Chain Reaction Book Excerpt:

James D. Watson When, in late March of 1953, Francis Crick and I came to write the first Nature paper describing the double helical structure of the DNA molecule, Francis had wanted to include a lengthy discussion of the genetic implications of a molecule whose struc ture we had divined from a minimum of experimental data and on theoretical argu ments based on physical principles. But I felt that this might be tempting fate, given that we had not yet seen the detailed evidence from King's College. Nevertheless, we reached a compromise and decided to include a sentence that pointed to the biological significance of the molecule's key feature-the complementary pairing of the bases. "It has not escaped our notice," Francis wrote, "that the specific pairing that we have postulated immediately suggests a possible copying mechanism for the genetic material." By May, when we were writing the second Nature paper, I was more confident that the proposed structure was at the very least substantially correct, so that this second paper contains a discussion of molecular self-duplication using templates or molds. We pointed out that, as a consequence of base pairing, a DNA molecule has two chains that are complementary to each other. Each chain could then act ". . . as a template for the formation on itself of a new companion chain, so that eventually we shall have two pairs of chains, where we only had one before" and, moreover, " ...

Molecular Biology

Molecular Biology
Author: Burton E. Tropp
Publsiher: Jones & Bartlett Publishers
Total Pages: 1097
Release: 2011-03-11
ISBN: 0763786632
Category: Science
Language: EN, FR, DE, ES & NL

Molecular Biology Book Excerpt:

"Molecular Biology: Genes to Proteins is a guide through the basic molecular processes and genetic phenomena of both prokaryotic and eukaryotic cells. Written for the undergraduate and first year graduate students within molecular biology or molecular genetics, the text has been updated with the latest data in the field. It incorporates a biochemical approach as well as a discovery approach that provides historical and experimental information within the context of the narrative."--Publisher.

Granular Neural Networks Pattern Recognition and Bioinformatics

Granular Neural Networks  Pattern Recognition and Bioinformatics
Author: Sankar K. Pal,Shubhra S. Ray,Avatharam Ganivada
Publsiher: Springer
Total Pages: 227
Release: 2017-05-02
ISBN: 331957115X
Category: Technology & Engineering
Language: EN, FR, DE, ES & NL

Granular Neural Networks Pattern Recognition and Bioinformatics Book Excerpt:

This book provides a uniform framework describing how fuzzy rough granular neural network technologies can be formulated and used in building efficient pattern recognition and mining models. It also discusses the formation of granules in the notion of both fuzzy and rough sets. Judicious integration in forming fuzzy-rough information granules based on lower approximate regions enables the network to determine the exactness in class shape as well as to handle the uncertainties arising from overlapping regions, resulting in efficient and speedy learning with enhanced performance. Layered network and self-organizing analysis maps, which have a strong potential in big data, are considered as basic modules,. The book is structured according to the major phases of a pattern recognition system (e.g., classification, clustering, and feature selection) with a balanced mixture of theory, algorithm, and application. It covers the latest findings as well as directions for future research, particularly highlighting bioinformatics applications. The book is recommended for both students and practitioners working in computer science, electrical engineering, data science, system design, pattern recognition, image analysis, neural computing, social network analysis, big data analytics, computational biology and soft computing.

Comprehensive Laboratory Manual of Life Sciences

Comprehensive Laboratory Manual of Life Sciences
Author: Mamta Baunthiyal,Indu Ravi,Jyoti Saxena
Publsiher: Scientific Publishers
Total Pages: 458
Release: 2019-12-19
ISBN: 9387991571
Category: Science
Language: EN, FR, DE, ES & NL

Comprehensive Laboratory Manual of Life Sciences Book Excerpt:

The present book ‘Comprehensive Laboratory Manual of Life Science’, deals with practical trends in modern biological sciences. It furnishes protocols on recent advances in biotechnological methods and aims to cover three most important aspects of this interdisciplinary stream; such as Microbiology, Biochemistry and Molecular biology. The book contains four sections: 1. Introduction: emphasizes on good laboratory practices and etiquettes for beginners; the do’s and don’ts of working in a laboratory, concepts and terminology, etc. 2. Instruments: Principle and Precautions: explores commonly used equipments employed in different experiments. 3. Experiments: is further divided into three parts: Microbiology with more than 70 experiments, Biochemistry with 62 and Molecular Biology having around 32 detailed protocols, accorded to make the readers proficient in the paramount disciplines of Bio Sciences and Biotechnology. 4. Appendix: at the end, a rather comprehensive section that concludes the book. This book is designed to meet the practical requirements of undergraduate and post graduate students of Life Science, Biotechnology, Microbiology, Biochemistry and Biochemical Engineering by providing worked out solution to the most commonly practiced experiments prescribed by majority of Indian Universities. The latest technological developments in the book will be appealing to the researchers and scientists

Mental Retardation and Developmental Delay

Mental Retardation and Developmental Delay
Author: Moyra Smith
Publsiher: Oxford University Press
Total Pages: 317
Release: 2006
ISBN: 0195174321
Category: Medical
Language: EN, FR, DE, ES & NL

Mental Retardation and Developmental Delay Book Excerpt:

This book makes readily available current knowledge on the subject of mental retardation and developmental delay and applies it to clinical medicine, providing information essential to neurologists, geneticists, physicians and pediatricians as they search for the causes of mental handicap in their patients. Chapters cover normal and abnormal brain structure, neurogenesis, and signal transduction before delving into a discussion of both the environmental factors that may lead to neurocognitive deficits and the cytogenetic, biochemical and molecular defects specifically associated with mental retardation. The text concludes with a clinically practical discussion of carrier detection, presymptomatic diagnosis, and treatment of various genetic diseases.

Hypercholesterolemia Hypocholesterolemia Hypertriglyceridemia in Vivo Kinetics

Hypercholesterolemia  Hypocholesterolemia  Hypertriglyceridemia  in Vivo Kinetics
Author: Claude L. Malmendier,P. Alaupovic,H. Bryan Brewer
Publsiher: Springer Science & Business Media
Total Pages: 454
Release: 2013-03-13
ISBN: 146845904X
Category: Medical
Language: EN, FR, DE, ES & NL

Hypercholesterolemia Hypocholesterolemia Hypertriglyceridemia in Vivo Kinetics Book Excerpt:

The past two decades have seen steady progress in our understanding of the pathogenesis of atherosclerosis. The role of low density lipoprotein (LOL) increase and of LOL receptor deficiency or malfunctions in familial hypercholesterolemia has been largely enlightened by the works of Brown and Goldstein. These authors postulated also that modification of LOL to a form recognized by the scavenger or acetyl-LOL receptor may be required for lipid loading of macrophage-derived foam cells in the lesions. A growing body of evidence suggests that oxidative modification of LOL could enhance its atherogenicity by its implication as a factor in the generation of foam cells. Thus, if the role of LOL in the pathogenesis of hypercholesterolemia was well established a great deal of information appears currently on new approaches such as the mechanisms leading to the accumulation of foam cells, the impact of LOL structural alterations, notably oxidation and the role of gene mutations of apolipoprotein Band/or LOL receptor The opening topic is devoted to these new avenues outlined in the field of hypercholesterolemia. The first part concerns the genetic aspects of atherosclerosis: mainly the genetics of apo 1 ipoprote ins , their transcriptional regulation, the amino acid mutations of the apo B gene and of the LOL receptor gene, the structural domains and the acylation sites of apoprotein B.

I A Novel Computational Methodology for Identifying RNA Minimum Sequence Requirements for Ligand Binding and Recognition

I  A Novel Computational Methodology for Identifying RNA Minimum Sequence Requirements for Ligand Binding and Recognition
Author: Peter C. Anderson
Publsiher: Unknown
Total Pages: 344
Release: 2007
ISBN: 1928374650XXX
Category: Electronic Book
Language: EN, FR, DE, ES & NL

I A Novel Computational Methodology for Identifying RNA Minimum Sequence Requirements for Ligand Binding and Recognition Book Excerpt: