Single Molecule Biology

Single Molecule Biology
Author: Alexander E. Knight
Publsiher: Academic Press
Total Pages: 368
Release: 2009-02-26
ISBN: 9780080921235
Category: Science
Language: EN, FR, DE, ES & NL

Single Molecule Biology Book Excerpt:

Single molecule techniques, including single molecule fluorescence, optical tweezers, and scanning probe microscopy, allow for the manipulation and measurement of single biological molecules within a live cell or in culture. These approaches, amongst the most exciting tools available in biology today, offer powerful new ways to elucidate biological function, both in terms of revealing mechanisms of action on a molecular level as well as tracking the behaviour of molecules in living cells. This book provides the first complete and authoritative treatment of this rapidly emerging field, explicitly from a biological perspective. The contents are organized by biological system or molecule. Each chapter discusses insights that have been revealed about their mechanism, structure or function by single molecule techniques. Among the topics covered are enzymes, motor proteins, membrane channels, DNA, ribozymes, cytoskeletal proteins, and other key molecules of current interest. An introduction by the editor provides a concise review of key principles and an historical overview. The last section discusses applications in molecular diagnostics and drug discovery. * Organized by biological system or molecule. * Each chapter discusses insights into mechanism of action, structure, and function * Covers enzymes, motor proteins, membrane channels, DNA, ribozymes, etc. * Includes an introduction to key principles and an historical overview. * Discusses applications in molecular diagnostics and drug discovery. * Provides an expert's perspective on future developments.

Biology at the Single Molecule Level

Biology at the Single Molecule Level
Author: S.H. Leuba,J. Zlatanova
Publsiher: Elsevier
Total Pages: 262
Release: 2001-12-17
ISBN: 9780080560618
Category: Science
Language: EN, FR, DE, ES & NL

Biology at the Single Molecule Level Book Excerpt:

This is the first book solely devoted to single-molecule biochemistry and molecular biology. Authors were selected on the basis of their contribution to this new and exciting field, and were asked to focus more on the biological problems that can be approached using single-molecule techniques rather than on the techniques per se. It is thought that such techniques will eventually dominate the physical characterization of biologically important macromolecules.

Single Molecule Spectroscopy in Chemistry Physics and Biology

Single Molecule Spectroscopy in Chemistry  Physics and Biology
Author: Astrid Graslund
Publsiher: Springer Science & Business Media
Total Pages: 596
Release: 2010-04-17
ISBN: 3642025986
Category: Molecular spectroscopy
Language: EN, FR, DE, ES & NL

Single Molecule Spectroscopy in Chemistry Physics and Biology Book Excerpt:

Written by the leading experts in the field, this book describes the development and current state of the art in single molecule spectroscopy. The application of this technique, which started 1989, in physics, chemistry and biosciences is displayed.

Single molecule Biophysics and Molecular Biology

Single molecule Biophysics and Molecular Biology
Author: Anonim
Publsiher: Unknown
Total Pages: 135
Release: 2000
ISBN: 1928374650XXX
Category: Electronic Book
Language: EN, FR, DE, ES & NL

Single molecule Biophysics and Molecular Biology Book Excerpt:

Single Molecule Analysis

Single Molecule Analysis
Author: Erwin J. G. Peterman,Gijs J. L. Wuite
Publsiher: Humana Press
Total Pages: 317
Release: 2011-09-15
ISBN: 9781617792816
Category: Science
Language: EN, FR, DE, ES & NL

Single Molecule Analysis Book Excerpt:

Life scientists believe that life is driven, directed, and shaped by biomolecules working on their own or in concert. It is only in the last few decades that technological breakthroughs in sensitive fluorescence microscopy and single-molecule manipulation techniques have made it possible to observe and manipulate single biomolecules and measure their individual properties. The methodologies presented in Single Molecule Techniques: Methods and Protocols are being applied more and more to the study of biologically relevant molecules, such as DNA, DNA-binding proteins, and motor proteins, and are becoming commonplace in molecular biophysics, biochemistry, and molecular and cell biology. The aim of Single Molecule Techniques: Methods and Protocols is to provide a broad overview of single-molecule approaches applied to biomolecules on the basis of clear and concise protocols, including a solid introduction to the most widely used single-molecule techniques, such as optical tweezers, single-molecule fluorescence tools, atomic force microscopy, magnetic tweezers, and tethered particle motion. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Single Molecule Techniques: Methods and Protocols serves as an ideal guide to scientists of all backgrounds and provides a broad and thorough overview of the exciting and still-emerging field of single-molecule biology.

Single Molecule Cellular Biophysics

Single Molecule Cellular Biophysics
Author: Mark C. Leake
Publsiher: Cambridge University Press
Total Pages: 135
Release: 2013-01-31
ISBN: 1139619349
Category: Science
Language: EN, FR, DE, ES & NL

Single Molecule Cellular Biophysics Book Excerpt:

Recent advances in single molecule science have presented a new branch of science: single molecule cellular biophysics, combining classical cell biology with cutting-edge single molecule biophysics. This textbook explains the essential elements of this new discipline, from the state-of-the-art single molecule techniques to real-world applications in unravelling the inner workings of the cell. Every effort has been made to ensure the text can be easily understood by students from both the physical and life sciences. Mathematical derivations are kept to a minimum whilst unnecessary biological terminology is avoided and text boxes provide readers from either background with additional information. 100 end-of-chapter exercises are divided into those aimed at physical sciences students, those aimed at life science students and those that can be tackled by students from both disciplines. The use of case studies and real research examples make this textbook indispensable for undergraduate students entering this exciting field.

Handbook of Single Molecule Biophysics

Handbook of Single Molecule Biophysics
Author: Peter Hinterdorfer,Antoine van Oijen
Publsiher: Springer Science & Business Media
Total Pages: 626
Release: 2009-12-24
ISBN: 9780387764979
Category: Science
Language: EN, FR, DE, ES & NL

Handbook of Single Molecule Biophysics Book Excerpt:

This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells.

Single Molecule Biophysics

Single Molecule Biophysics
Author: Tamiki Komatsuzaki,Masaru Kawakami,Satoshi Takahashi,Haw Yang,Robert J. Silbey
Publsiher: John Wiley & Sons
Total Pages: 512
Release: 2011-11-16
ISBN: 111813138X
Category: Science
Language: EN, FR, DE, ES & NL

Single Molecule Biophysics Book Excerpt:

Discover the experimental and theoretical developments inoptical single-molecule spectroscopy that are changing the ways wethink about molecules and atoms The Advances in Chemical Physics series provides thechemical physics field with a forum for critical, authoritativeevaluations of advances in every area of the discipline. Thislatest volume explores the advent of optical single-moleculespectroscopy, and how atomic force microscopy has empowered novelexperiments on individual biomolecules, opening up new frontiers inmolecular and cell biology and leading to new theoreticalapproaches and insights. Organized into two parts—oneexperimental, the other theoretical—this volume exploresadvances across the field of single-molecule biophysics, presentingnew perspectives on the theoretical properties of atoms andmolecules. Single-molecule experiments have provided freshperspectives on questions such as how proteins fold to specificconformations from highly heterogeneous structures, how signaltransductions take place on the molecular level, and how proteinsbehave in membranes and living cells.This volume is designed tofurther contribute to the rapid development of single-moleculebiophysics research. Filled with cutting-edge research reported in a cohesive mannernot found elsewhere in the literature, each volume of theAdvances in Chemical Physics series serves as the perfectsupplement to any advanced graduate class devoted to the study ofchemical physics.

Single Molecule Biophysics and Poisson Process Approach to Statistical Mechanics

Single Molecule Biophysics and Poisson Process Approach to Statistical Mechanics
Author: Susanta K Sarkar
Publsiher: Morgan & Claypool Publishers
Total Pages: 74
Release: 2016-04-01
ISBN: 1681741806
Category: Science
Language: EN, FR, DE, ES & NL

Single Molecule Biophysics and Poisson Process Approach to Statistical Mechanics Book Excerpt:

This is an overview of single molecule physics, the study of both equilibrium and non-equilibrium properties at the single molecule level. It begins with an introduction to this fascinating science and includes a chapter on how to build the most popular instrument for single molecule biophysics, the total internal reflection fluorescence (TIRF) microscope. It concludes with the Poisson process approach to statistical mechanics, explaining how to relate the process to diverse areas and see how data analysis and error bars are integral parts of science.

Single molecule Studies of Proteins

Single molecule Studies of Proteins
Author: Andres F. Oberhauser
Publsiher: Springer Science & Business Media
Total Pages: 274
Release: 2012-11-09
ISBN: 1461449219
Category: Science
Language: EN, FR, DE, ES & NL

Single molecule Studies of Proteins Book Excerpt:

In Single Molecule Studies of Proteins, expert researchers discuss the successful application of single-molecule techniques to a wide range of biological events, such as the imaging and mapping of cell surface receptors, the analysis of the unfolding and folding pathways of single proteins, the analysis interaction forces between biomolecules, the study of enzyme catalysis or the visualization of molecular motors in action. The chapters are aimed at established investigators and post-doctoral researchers in the life sciences wanting to pursue research in the various areas in which single-molecule approaches are important; this volume also remains accessible to advanced graduate students seeking similar research goals.

Single molecule Techniques

Single molecule Techniques
Author: Paul R. Selvin,Taekjip Ha
Publsiher: CSHL Press
Total Pages: 511
Release: 2008
ISBN: 087969775X
Category: Science
Language: EN, FR, DE, ES & NL

Single molecule Techniques Book Excerpt:

Geared towards research scientists in structural and molecular biology, biochemistry, and biophysics, this manual will be useful to all who are interested in observing, manipulating and elucidating the molecular mechanisms and discrete properties of macromolecules.

Focussed Issue Single molecule Biophysics and Molecular Biology

Focussed Issue  Single molecule Biophysics and Molecular Biology
Author: S. Leuba
Publsiher: Unknown
Total Pages: 140
Release: 2000
ISBN: 1928374650XXX
Category: Electronic Book
Language: EN, FR, DE, ES & NL

Focussed Issue Single molecule Biophysics and Molecular Biology Book Excerpt:

Single Molecule Science

Single Molecule Science
Author: Krishnarao Appasani,Raghu Kiran Appasani
Publsiher: Unknown
Total Pages: 172
Release: 2022-05-11
ISBN: 110853449X
Category: Medical
Language: EN, FR, DE, ES & NL

Single Molecule Science Book Excerpt:

A comprehensive volume that brings together authoritative overviews of single molecule science techniques from a biological perspective.

Single Molecule Tools Part B Super Resolution Particle Tracking Multiparameter and Force Based Methods

Single Molecule Tools  Part B  Super Resolution  Particle Tracking  Multiparameter  and Force Based Methods
Author: Anonim
Publsiher: Academic Press
Total Pages: 720
Release: 2010-07-09
ISBN: 9780123814838
Category: Science
Language: EN, FR, DE, ES & NL

Single Molecule Tools Part B Super Resolution Particle Tracking Multiparameter and Force Based Methods Book Excerpt:

Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part B: Super-Resolution, Particle Tracking, Multiparameter, and Force Based Methods captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools Methods included are useful to both established researchers and newcomers to the field Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines

Single Molecule Tools Part A Fluorescence Based Approaches

Single Molecule Tools  Part A  Fluorescence Based Approaches
Author: Anonim
Publsiher: Academic Press
Total Pages: 528
Release: 2010-08-17
ISBN: 9780080959276
Category: Science
Language: EN, FR, DE, ES & NL

Single Molecule Tools Part A Fluorescence Based Approaches Book Excerpt:

Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part A: Fluorescence Based Approaches captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools Methods included are useful to both established researchers and newcomers to the field Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines

Single Molecules and Nanotechnology

Single Molecules and Nanotechnology
Author: Rudolf Rigler,H. Vogel
Publsiher: Springer Science & Business Media
Total Pages: 318
Release: 2007-12-07
ISBN: 3540739246
Category: Technology & Engineering
Language: EN, FR, DE, ES & NL

Single Molecules and Nanotechnology Book Excerpt:

This book focuses on recent advances in the rapidly evolving field of single molecule research. These advances are of importance for the investigation of biopolymers and cellular biochemical reactions, and are essential to the development of quantitative biology. Written by leading experts in the field, the articles cover a broad range of topics, including quantum photonics of organic dyes and inorganic nanoparticles and monitoring of single molecule (enzymatic) reactions.

Handbook of Single Molecule Biophysics

Handbook of Single Molecule Biophysics
Author: Peter Hinterdorfer,Antoine van Oijen
Publsiher: Springer
Total Pages: 626
Release: 2016-08-23
ISBN: 9781493939190
Category: Science
Language: EN, FR, DE, ES & NL

Handbook of Single Molecule Biophysics Book Excerpt:

This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells.

Single Molecule Enzymology Fluorescence Based and High Throughput Methods

Single Molecule Enzymology  Fluorescence Based and High Throughput Methods
Author: Anonim
Publsiher: Academic Press
Total Pages: 616
Release: 2016-10-28
ISBN: 0128095474
Category: Medical
Language: EN, FR, DE, ES & NL

Single Molecule Enzymology Fluorescence Based and High Throughput Methods Book Excerpt:

Single-Molecule Enzymology, Part A, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in single-molecule enzymology Contains sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion

Single Molecule Enzymology Nanomechanical Manipulation and Hybrid Methods

Single Molecule Enzymology  Nanomechanical Manipulation and Hybrid Methods
Author: Anonim
Publsiher: Academic Press
Total Pages: 484
Release: 2017-01-03
ISBN: 0128095032
Category: Science
Language: EN, FR, DE, ES & NL

Single Molecule Enzymology Nanomechanical Manipulation and Hybrid Methods Book Excerpt:

Single-Molecule Enzymology, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in single-molecule enzymology Contains sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion

Biophysics of DNA Protein Interactions

Biophysics of DNA Protein Interactions
Author: Mark C. Williams,L. James Maher, III
Publsiher: Springer Science & Business Media
Total Pages: 350
Release: 2010-10-05
ISBN: 0387928081
Category: Science
Language: EN, FR, DE, ES & NL

Biophysics of DNA Protein Interactions Book Excerpt:

Depite the rapid expansion of the field of biophysics, there are very few books that comprehensively treat specific topics in this area. Recently, the field of single molecule biophysics has developed very quickly, and a few books specifically treating single molecule methods are beginning to appear. However, the promise of single molecule biophysics is to contribute to the understanding of specific fields of biology using new methods. This book would focus on the specific topic of the biophysics of DNA-protein interactions, and would include the use of new approaches, including both bulk methods as well as single molecule methods. This would make the book attractive to anyone working in the general area of DNA-protein interactions, which is of course a much wider market than just single molecule biophysicists or even biophysicists. The subject of the book will be the biophysics of DNA-protein interactions, and will include new methods and results that describe the physical mechanism by which proteins interact with DNA. For example, there has been much recent work on the mechanism by which proteins search for specific binding sites on DNA. A few chapters will be devoted to experiments and theory that shed light on this important problem. We will also cover proteins that alter DNA properties to facilitate interactions important for transcription or replication. Another section of the book will cover the biophysical mechanism by which motor proteins interact with DNA. Finally, we will cover larger protein-DNA complexes, such as replication forks, recombination complexes, DNA repair interactions, and their chromatin context.