Synthetic Biology A Lab Manual

Author by : Forster Anthony C
Languange : en
Publisher by : World Scientific
Format Available : PDF, ePub, Mobi
Total Read : 94
Total Download : 371
File Size : 41,7 Mb
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Description : Synthetic Biology: A Lab Manual is the first manual for laboratory work in the new and rapidly expanding field of synthetic biology. Aimed at non-specialists, it details protocols central to synthetic biology in both education and research. In addition, it provides all the information that teachers and students from high schools and tertiary institutions need for a colorful lab course in bacterial synthetic biology using chromoproteins and designer antisense RNAs. As a bonus, practical material is provided for students of the annual international Genetically Engineered Machine (iGEM) competition. The manual is based upon a highly successful course at Sweden's Uppsala University and is coauthored by one of the pioneers of synthetic biology and two bioengineering postgraduate students.An inspiring foreword is written by another pioneer in the field, Harvard's George Church: “Synthetic biology is to early recombinant DNA as a genome is to a gene. Is there anything that SynBio will not impact? There was no doubt that the field of SynBio needed ‘A Lab Manual’ such as the one that you now hold in your hands.”


Biobuilder

Author by : Natalie Kuldell PhD.
Languange : en
Publisher by : "O'Reilly Media, Inc."
Format Available : PDF, ePub, Mobi
Total Read : 88
Total Download : 212
File Size : 55,7 Mb
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Description : Today’s synthetic biologists are in the early stages of engineering living cells to help treat diseases, sense toxic compounds in the environment, and produce valuable drugs. With this manual, you can be part of it. Based on the BioBuilder curriculum, this valuable book provides open-access, modular, hands-on lessons in synthetic biology for secondary and post-secondary classrooms and laboratories. It also serves as an introduction to the field for science and engineering enthusiasts. Developed at MIT in collaboration with award-winning high school teachers, BioBuilder teaches the foundational ideas of the emerging synthetic biology field, as well as key aspects of biological engineering that researchers are exploring in labs throughout the world. These lessons will empower teachers and students to explore and be part of solving persistent real-world challenges. Learn the fundamentals of biodesign and DNA engineering Explore important ethical issues raised by examples of synthetic biology Investigate the BioBuilder labs that probe the design-build-test cycle Test synthetic living systems designed and built by engineers Measure several variants of an enzyme-generating genetic circuit Model "bacterial photography" that changes a strain’s light sensitivity Build living systems to produce purple or green pigment Optimize baker’s yeast to produce ?-carotene


Synthetic Biology

Author by : Christopher Voigt
Languange : en
Publisher by : Academic Press
Format Available : PDF, ePub, Mobi
Total Read : 40
Total Download : 279
File Size : 49,8 Mb
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Description : Synthetic biology encompasses a variety of different approaches, methodologies and disciplines, and many different definitions exist. This Volume of Methods in Enzymology has been split into 2 Parts and covers topics such as Measuring and Engineering Central Dogma Processes, Mathematical and Computational Methods and Next-Generation DNA Assembly and Manipulation. Encompasses a variety of different approaches, methodologies and disciplines Split into 2 parts and covers topics such as measuring and engineering central dogma processes, mathematical and computational methods and next-generation DNA assembly and manipulation


Synthetic Biology

Author by : Christopher Voigt
Languange : en
Publisher by : Academic Press
Format Available : PDF, ePub, Mobi
Total Read : 47
Total Download : 351
File Size : 53,6 Mb
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Description : Synthetic biology encompasses a variety of different approaches, methodologies and disciplines, and many different definitions exist. This Volume of Methods in Enzymology has been split into 2 Parts and covers topics such as Measuring and Engineering Central Dogma Processes, Mathematical and Computational Methods and Next-Generation DNA Assembly and Manipulation. Encompasses a variety of different approaches, methodologies and disciplines Split into 2 parts and covers topics such as measuring and engineering central dogma processes, mathematical and computational methods and next-generation DNA assembly and manipulation


Molecular Biology Techniques

Author by : Heather Miller
Languange : en
Publisher by : Academic Press
Format Available : PDF, ePub, Mobi
Total Read : 35
Total Download : 458
File Size : 48,9 Mb
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Description : This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions


Plant Biotechnology And Molecular Biology A Laboratory Manual

Author by : M.S. Punia
Languange : en
Publisher by : Scientific Publishers
Format Available : PDF, ePub, Mobi
Total Read : 26
Total Download : 765
File Size : 44,7 Mb
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Description : The book, “A Laboratory Manual of Plant Biotechnology and Molecular Biology” comprises of workable laboratory protocols for a large number of techniques related to plant biotechnology, genetic engineering and molecular biology. This includes plant cell and tissue culture, callus and suspension culture, anther culture, ovule culture, embryo culture, Cryopreservation, Isolation of Plant protoplasts, Protoplast culture and regeneration, production of somatic hybrids through protoplast fusion, gene transformation using Agrobacterium as vector, direct gene transfer using biolistic gun, Isolation of plant and organells DNA, construction and screening of genomic DNA libraries, Molecular markers like RFLP, RAPD, SCARS and CAPS, DNA sequencing, RNA isolation and northern blotting, Isolation of proteins and western blotting etc. The manual is prepared with the objective to cater the needs of post- graduate students as well as for scientists working in the disciplines of Plant Breeding, Genetics, Botany, Plant physiology, Biochemistry, Plant Biotechnology, Molecular Biology etc. It gives an update on some well established methods and presents reliable protocols.


Elements Of General And Biological Chemistry Laboratory Manual

Author by : John R. Holum
Languange : en
Publisher by : Wiley
Format Available : PDF, ePub, Mobi
Total Read : 61
Total Download : 903
File Size : 45,7 Mb
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Description : This updated editon explains recent advances in environmental studies and in the molecular basis of life. Suitable for students interested in the health care field as well as those who want to know how nature and human life work at the molecular level, the book begins by providing readers with a solid background in formulas, structures, equations, solutions and equilibria. A number of topics are introduced early, such as molarity, and are discussed in more detail in later chapters. Each chapter contains a summary as well as review exercises.


Brutes Or Angels

Author by : James T. Bradley
Languange : en
Publisher by : University of Alabama Press
Format Available : PDF, ePub, Mobi
Total Read : 20
Total Download : 409
File Size : 47,7 Mb
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Description : "With stem cell research, Dolly the cloned sheep, in vitro fertilization, age retardation, and pharmaceutical mind enhancement, humankind is now faced with decisions that it has never before had to consider. The thoughtfulness, or lack of it, that we bring to those decisions will largely determine the future character of the living world. Brutes or Angels will facilitate informed choice making about the personal use of biotechnologies and the formulation of public policies governing their development and use. Ten biotechnologies that impact humans are considered: stem cell research, embryo selection, human genomics, gene therapies, human reproductive cloning, age retardation, cognition enhancement, the engineering of nonhuman organisms, nanobiology, and synthetic biology. With deft and assured use of metaphors, analogies, diagrams, and photographs, James T. Bradley introduces important biological principles and the basic procedures used in biotechnology. Various ethical issues--personhood, personal identity, privacy, ethnic discrimination, distributive justice, authenticity and human nature, and the significance of mortality in the human life cycle--are presented in a clear and unbiased manner. Personal reflection and group dialogue are encouraged by questions at the end of each chapter, making this book not only a general guide to better informed and nuanced thinking on these complex and challenging topics but also an appropriate text for bioethics courses in university science departments and for adult education classes." -- Publisher's description.


Biology Laboratory Manual

Author by : Sylvia S. Mader
Languange : en
Publisher by : McGraw-Hill Science, Engineering & Mathematics
Format Available : PDF, ePub, Mobi
Total Read : 81
Total Download : 741
File Size : 41,7 Mb
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Description : Mader includes revised coverage of animal behaviour and ecology as well as a wealth of new focus boxes which highlight topics of high interest and relate biology to everyday life. This text is linked to a web site offering extended chapter outlines.


Kinanthropometry And Exercise Physiology Laboratory Manual

Author by : Roger Eston
Languange : en
Publisher by : Taylor & Francis
Format Available : PDF, ePub, Mobi
Total Read : 25
Total Download : 401
File Size : 41,6 Mb
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Description : Developed as a key resource for both lecturers and students of kinanthropometry, sports science, human movement and exercise physiology, this laboratory manual provides help with the planning and conduct of class practicals; comprehensive theoretical background for each topic so that the reader can easily place the subject in context without the need for extensive literature reviews; original laboratory practicals and suggestions for student activities; a chapter on statistical analysis which promotes the proper use of common statistical techniques for analysing data obtained on human subjects as well as helping to avoid common abuses of basic statistical tools; and self-standing chapters which are independent of each other enabling the reader to pick out topics of interest in any order.