Synthetic Biology Part B

Author by : Chris Voigt
Languange : en
Publisher by : Academic Press
Format Available : PDF, ePub, Mobi
Total Read : 29
Total Download : 661
File Size : 41,5 Mb
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Description : Synthetic biology encompasses a variety of different approaches, methodologies and disciplines, and many different definitions exist. This Volume of Methods in Enzymology has been split into 2 Parts and covers topics such as Measuring and Engineering Central Dogma Processes, Mathematical and Computational Methods and Next-Generation DNA Assembly and Manipulation. Encompasses a variety of different approaches, methodologies and disciplines Split into 2 parts and covers topics such as measuring and engineering central dogma processes, mathematical and computational methods and next-generation DNA assembly and manipulation


Synthetic Biology

Author by : Christopher Voigt
Languange : en
Publisher by : Academic Press
Format Available : PDF, ePub, Mobi
Total Read : 17
Total Download : 713
File Size : 41,8 Mb
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Description : Synthetic biology encompasses a variety of different approaches, methodologies and disciplines, and many different definitions exist. This Volume of Methods in Enzymology has been split into 2 Parts and covers topics such as Measuring and Engineering Central Dogma Processes, Mathematical and Computational Methods and Next-Generation DNA Assembly and Manipulation. Encompasses a variety of different approaches, methodologies and disciplines Split into 2 parts and covers topics such as measuring and engineering central dogma processes, mathematical and computational methods and next-generation DNA assembly and manipulation


Chemical And Synthetic Biology Approaches To Understand Cellular Functions

Author by :
Languange : en
Publisher by : Academic Press
Format Available : PDF, ePub, Mobi
Total Read : 34
Total Download : 406
File Size : 42,5 Mb
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Description : Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part A, Volume 621, the latest release in the Methods in Enzymology series, highlights new advances in the field, with this volume covering Site-directed ethylation of membrane proteins for measuring conformational transitions in lipid bilayers, the Design and synthesis of fluorescent activity probes for protein phosphatases, Stains, Utilizing split-nanoLuc fragments as luminescent probes for protein solubility in living cells, SH2-domain based sensor for intracellular recognition of sulfo-tyrosine, DNA-encoded immunoglobulins for detection of parasites, An engineered TEV protease – calmodulin fusion based sensor for neuronal calcium recording, and much more. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series Includes the latest information on methods to measure ubiquitin chain length and linkage and genetic approaches to study the yeast ubiquitin system, amongst other timely topics


Biobuilder

Author by : Natalie Kuldell PhD.
Languange : en
Publisher by : "O'Reilly Media, Inc."
Format Available : PDF, ePub, Mobi
Total Read : 83
Total Download : 356
File Size : 41,5 Mb
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Description : Today’s synthetic biologists are in the early stages of engineering living cells to help treat diseases, sense toxic compounds in the environment, and produce valuable drugs. With this manual, you can be part of it. Based on the BioBuilder curriculum, this valuable book provides open-access, modular, hands-on lessons in synthetic biology for secondary and post-secondary classrooms and laboratories. It also serves as an introduction to the field for science and engineering enthusiasts. Developed at MIT in collaboration with award-winning high school teachers, BioBuilder teaches the foundational ideas of the emerging synthetic biology field, as well as key aspects of biological engineering that researchers are exploring in labs throughout the world. These lessons will empower teachers and students to explore and be part of solving persistent real-world challenges. Learn the fundamentals of biodesign and DNA engineering Explore important ethical issues raised by examples of synthetic biology Investigate the BioBuilder labs that probe the design-build-test cycle Test synthetic living systems designed and built by engineers Measure several variants of an enzyme-generating genetic circuit Model "bacterial photography" that changes a strain’s light sensitivity Build living systems to produce purple or green pigment Optimize baker’s yeast to produce ?-carotene


Synthetic Biology

Author by : Markus Schmidt
Languange : en
Publisher by : Springer Science & Business Media
Format Available : PDF, ePub, Mobi
Total Read : 60
Total Download : 629
File Size : 52,9 Mb
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Description : Synthetic biology is becoming one of the most dynamic new fields of biology, with the potential to revolutionize the way we do biotechnology today. By applying the toolbox of engineering disciplines to biology, a whole set of potential applications become possible ranging very widely across scientific and engineering disciplines. Some of the potential benefits of synthetic biology, such as the development of low-cost drugs or the production of chemicals and energy by engineered bacteria are enormous. There are, however, also potential and perceived risks due to deliberate or accidental damage. Also, ethical issues of synthetic biology just start being explored, with hardly any ethicists specifically focusing on the area of synthetic biology. This book will be the first of its kind focusing particularly on the safety, security and ethical concerns and other relevant societal aspects of this new emerging field. The foreseen impact of this book will be to stimulate a debate on these societal issues at an early stage. Past experiences, especially in the field of GM-crops and stem cells, have shown the importance of an early societal debate. The community and informed stakeholders recognize this need, but up to now discussions are fragmentary. This book will be the first comprehensive overview on relevant societal issues of synthetic biology, setting the scene for further important discussions within the scientific community and with civil society.


Chemical Synthetic Biology

Author by : Pier Luigi Luisi
Languange : en
Publisher by : John Wiley & Sons
Format Available : PDF, ePub, Mobi
Total Read : 82
Total Download : 393
File Size : 45,6 Mb
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Description : Chemistry plays a very important role in the emerging field of synthetic biology. In particular, chemical synthetic biology is concerned with the synthesis of chemical structures, such as proteins, that do not exist in nature. With contributions from leading international experts, Chemical Synthetic Biology shows how chemistry underpins synthetic biology. The book is an essential guide to this fascinating new field, and will find a place on the bookshelves of researchers and students working in synthetic chemistry, synthetic and molecular biology, bioengineering, systems biology, computational genomics, and bioinformatics.


Synthetic Aesthetics

Author by : Alexandra Daisy Ginsberg
Languange : en
Publisher by : MIT Press
Format Available : PDF, ePub, Mobi
Total Read : 58
Total Download : 478
File Size : 52,5 Mb
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Description : As synthetic biology transforms living matter into a medium for making, what is the role of design and its associated values?


Guide To Yeast Genetics And Molecular Biology

Author by : Christine Guthrie
Languange : en
Publisher by : Gulf Professional Publishing
Format Available : PDF, ePub, Mobi
Total Read : 80
Total Download : 741
File Size : 51,5 Mb
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Description : Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast. Key Features * Basic Methods in Yeast Genetics * Physical and genetic mapping * Making and recovering mutants * Cloning and Recombinant DNA Methods * High-efficiency transformation * Preparation of yeast artificial chromosome vectors * Basic Methods of Cell Biology * Immunomicroscopy * Protein targeting assays * Biochemistry of Gene Expression * Vectors for regulated expression * Isolation of labeled and unlabeled DNA, RNA, and protein


Developing Biofuel Bioprocesses Using Systems And Synthetic Biology

Author by : Sylvia M. Clay
Languange : en
Publisher by : Springer Science & Business Media
Format Available : PDF, ePub, Mobi
Total Read : 35
Total Download : 225
File Size : 50,9 Mb
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Description : Advances in technological and analytical methods have fostered rapid growth of systems biology and synthetic biology. There continues to be rapid changes and discoveries in both fields with a small number of recent peer-reviewed reviews indicating some of the relationships between systems biology and synthetic biology. This proposed SpringerBrief will cover core concepts of systems biology and synthetic biology and illustrate the implementation of associated research methodologies for an integrated approach to specifically address engineering microorganisms for biofuel production.​


Basic Methods In Molecular Biology

Author by : Leonard Davis
Languange : en
Publisher by : Elsevier
Format Available : PDF, ePub, Mobi
Total Read : 25
Total Download : 840
File Size : 50,9 Mb
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Description : Basic Methods in Molecular Biology discusses the heart of the most recent revolution in biology—the development of the technology of genetics. The achievements in this field have simply changed what biologists do and, perhaps even more important, the way they think. Moreover, never before have scientists from such a broad range of disciplines rushed into such a small and slightly arcane field to learn and carry off a bit of the technology. This book comprises 21 chapters, opening with three introductory ones that discuss the basics of molecular biology; the tools of the molecular biologist; and general preparations, procedures, and considerations for use of the book. The following chapters then discuss cloning vectors and bacterial cells; preparation of DNA from eukaryotic cells; probing nucleic acids; plasmid DNA preparation; DNA restriction fragment preparation; purification of DNA; and preparation and analysis of RNA from eukaryotic cells. Other chapters cover preparation of DNA from bacteriophage clones; cloning DNA from the eukaryotic genome; subcloning into plasmids; M13 cloning and sequencing; further characterization of cloned DNA; transfection of mammalian cells in culture; protein methods; general methods; and specialized methods. This book will be of interest to practitioners in the fields of biology and molecular genetics.