Understanding Light Microscopy

Author by : Jeremy Sanderson
Languange : en
Publisher by : Wiley
Format Available : PDF, ePub, Mobi
Total Read : 18
Total Download : 721
File Size : 43,5 Mb
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Description : Introduces readers to the enlightening world of the modern light microscope There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades. Written to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, Understanding Light Microscopy keeps mathematical formulae to a minimum—containing and explaining them within boxes in the text. Chapters provide in-depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected-light, polarised-light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP & FRET; super-resolution techniques; biological and materials specimen preparation; and more. Gives a didactic introduction to the light microscope Encourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility Features full-colour illustrations and workable practical protocols Understanding Light Microscopy is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.


Understanding The Light Microscope

Author by : D. J. Goldstein
Languange : en
Publisher by : Academic Press
Format Available : PDF, ePub, Mobi
Total Read : 81
Total Download : 630
File Size : 41,7 Mb
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Description : Understanding the Light Microscope consists of four original computer programs with an explanatory book. Author Dan Goldstein says using the programs can teach aspects of microscopy and diffraction often missed from formal courses, adding, "... what one non-mathematician has created should not be beyond the understanding of others!" The book aims to provide understanding at a level deeper than customary in existing texts and in a form accessible to microscope users, particularly biologists. It covers simple ray optics, the aberrations of "real" (thick) lenses, polarized light, and the influence of diffraction on imaging. The book can be read alone, but appreciation of its contents is greatly enhanced when used in conjunction with the programs. D J Goldstein was a Nuffield Dominion Travelling Fellow at Oxford University and a visiting Professor in Pittsburgh. He taught at the Universities of the Witwatersrand (Johannesburg, South Africa) and Sheffield (UK) while publishing research in embryology, histology, immunology, histochemistry and microscopy. Since retiring in 1989 as Reader in Anatomy at Sheffield University, he has been an independent research worker in biomedical science. Key Features * Aids insight into microscope operation and imitations * The approach is non-mathematical, yet in-depth * Enables lecture time to be replaced by learning assignments * Includes a help function for all four programs * The programs have been tried and tested by 2nd and 3rd year biomedical undergraduates


A Practical Guide To Optical Microscopy

Author by : John Girkin
Languange : en
Publisher by : CRC Press
Format Available : PDF, ePub, Mobi
Total Read : 95
Total Download : 711
File Size : 42,7 Mb
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Description : Optical microscopy is used in a vast range of applications ranging from materials engineering to in vivo observations and clinical diagnosis, and thanks to the latest advances in technology, there has been a rapid growth in the number of methods available. This book is aimed at providing users with a practical guide to help them select, and then use, the most suitable method for their application. It explores the principles behind the different forms of optical microscopy, without the use of complex maths, to provide an understanding to help the reader utilise a specific method and then interpret the results. Detailed physics is provided in boxed sections, which can be bypassed by the non-specialist. It is an invaluable tool for use within research groups and laboratories in the life and physical sciences, acting as a first source for practical information to guide less experienced users (or those new to a particular methodology) on the range of techniques available. Features: The first book to cover all current optical microscopy methods for practical applications Written to be understood by a non-optical expert with inserts to provide the physical science background Brings together conventional widefield and confocal microscopy, with advanced non-linear and super resolution methods, in one book


Fundamentals Of Light Microscopy

Author by : Michael Spencer
Languange : en
Publisher by : CUP Archive
Format Available : PDF, ePub, Mobi
Total Read : 73
Total Download : 537
File Size : 46,6 Mb
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Description : Dr Spencer's account gives a concise but rigorous explanation of the principles underlying the various forms of light microscopy.


Introduction To Optical Microscopy

Author by : Jerome Mertz
Languange : en
Publisher by : Cambridge University Press
Format Available : PDF, ePub, Mobi
Total Read : 59
Total Download : 551
File Size : 41,5 Mb
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Description : Presents a fully updated, self-contained textbook covering the core theory and practice of both classical and modern optical microscopy techniques.


Light Microscopy

Author by : David J. Rawlins
Languange : en
Publisher by : Taylor & Francis
Format Available : PDF, ePub, Mobi
Total Read : 27
Total Download : 774
File Size : 41,6 Mb
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Description : This is a straightforward and comprehensive guide to the practical use of the light microscope for the examination of biological specimens. Written in an informal style, it aims to be a readable explanation of all the types of light microscopy currently in use in the modern biological laboratory. It is designed to be used at the bench, next to the microscope. Theoretical explanations are kept to the minimum necessary to support the practical information.


Light Microscopy Of Carbon Steels

Author by : Leonard Ernest Samuels
Languange : en
Publisher by : ASM International
Format Available : PDF, ePub, Mobi
Total Read : 44
Total Download : 688
File Size : 52,5 Mb
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Description :


Light And Video Microscopy

Author by : Randy O. Wayne
Languange : en
Publisher by : Academic Press
Format Available : PDF, ePub, Mobi
Total Read : 84
Total Download : 229
File Size : 48,7 Mb
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Description : The purpose of this book is to provide the most comprehensive, easy-to-use, and informative guide on light microscopy. Light and Video Microscopy will prepare the reader for the accurate interpretation of an image and understanding of the living cell. With the presentation of geometrical optics, it will assist the reader in understanding image formation and light movement within the microscope. It also provides an explanation of the basic modes of light microscopy and the components of modern electronic imaging systems and guides the reader in determining the physicochemical information of living and developing cells, which influence interpretation. Brings together mathematics, physics, and biology to provide a broad and deep understanding of the light microscope Clearly develops all ideas from historical and logical foundations Laboratory exercises included to assist the reader with practical applications Microscope discussions include: bright field microscope, dark field microscope, oblique illumination, phase-contrast microscope, photomicrography, fluorescence microscope, polarization microscope, interference microscope, differential interference microscope, and modulation contrast microscope


Coherent Light Microscopy

Author by : Pietro Ferraro
Languange : en
Publisher by : Springer Science & Business Media
Format Available : PDF, ePub, Mobi
Total Read : 98
Total Download : 913
File Size : 52,5 Mb
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Description : This book deals with the latest achievements in the field of optical coherent microscopy. While many other books exist on microscopy and imaging, this book provides a unique resource dedicated solely to this subject. Similarly, many books describe applications of holography, interferometry and speckle to metrology but do not focus on their use for microscopy. The coherent light microscopy reference provided here does not focus on the experimental mechanics of such techniques but instead is meant to provide a users manual to illustrate the strengths and capabilities of developing techniques. The areas of application of this technique are in biomedicine, medicine, life sciences, nanotechnology and materials sciences.


Confocal Microscopy For Biologists

Author by : Alan R. Hibbs
Languange : en
Publisher by : Springer Science & Business Media
Format Available : PDF, ePub, Mobi
Total Read : 60
Total Download : 444
File Size : 48,7 Mb
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Description : There has been a great upsurge in interest in light microscopy in recent years due to the advent of a number of significant advances in microscopy, one of the most important of which is confocal microscopy. Confocal microscopy has now become an important research tool, with a large number of new fluorescent dyes becoming available in the past few years, for probing your pet structure or molecule within fixed or living cell or tissue sampies. Many of the people interested in using confocal microscopy to further their research do not have a background in microscopy or even cell biology and so not only do they find considerable difficulty in obtaining satisfactory results with a confocal microscope, but they may be mislead by how data is being presented. This book is intended to teach you the basic concepts ofmicroscopy, fluorescence, digital imaging and the principles of confocal microscopy so that you may take full advantage ofthe excellent confocal microscopes now available. This book is also an excellent reference source for information related to confocal microscopy for both beginners and the more advanced users. For example, do you need to know the optimal pinhole size for a 63x 1. 4 NA lens? Do you need to know the fluorescence emission spectrum of Alexa 568? Access to the wealth of practical information in this book is made easier by using both the detailed index and the extensive glossary.